内参抗体GAPDH (Internal)
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Species Cross-reactivity H
Key Application ELISA, WB
Host Mouse
Antibody type Monoclonal antibody
Purified method Affinity purified
Isotype IgG1
Molecular Weight 35kDa
Sensitivity This antibody detects endogenous levels GAPDH and does not cross-react with related proteins.
Immunogen Purified recombinant human GAPDH protein fragments expressed in E.coli.
UniProt Number P04406
GeneBank ID NM_002046
Subcellular location Cytoplasm; Nucleus
Formulation Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.2% sodium azide, 50%,glycerol
Altername G3PD; GAPD; MGC88685
Gene_symbol G3PD; GAPD
UniPort summary Function Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Entrez summary The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The enzyme exists as a tetramer of identical chains. Many pseudogenes similar to this locus are present in the human genome.
Application Images :
Western blot detection of GAPDH(Internal) antibody in K562,Jurkat&Hela cell lysates using GAPDH(Internal) antibody (1:1000 diluted). Predicted band size: 35KDa Observed band size: 35KDa
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