INSERT
基本信息
产品名称：
INSERT
英文名称：
HIV 1+2 Ab / P24 Ag EIA DIRECTION INSERT
国产/进口：
产地/品牌：
型号：
参考报价：
唐丽娅13701223090
销售商：
总点击数：
1516
更新日期：
2007-03-09
产品类别：
性能参数
HIV 1+2 Ab / P24 Ag EIA DIRECTION INSERT
The HIV 1+2 Ab / P24 Ag EIA is a qualitative enzyme immunoassay for the in vitro detection of HIV infection, based on the detection of antibodies to HIV 1 and HIV 2 and the P24 antigen in human serum or plasma. This assay has the advantage of being sensitive,specific,simple,and fast. It has great significance in the diagnosis of HIV infection / screen for donated blood to prevent transmission of HIV to recipients and as an aid in clinical diagnosis of HIV-related infections.
REAGENTS： 96 tests
1. Microplate 96 wells
2. Negative Control 1 ml ×1
3. Positive Control 1 ml ×1
4. P24 Antigen Control 1 ml ×1
5. Conjugate 12 ml ×1
6. Substrate Diluent 6 ml ×1
7. TMB Buffer 6 ml ×1
8. 20×Wash Buffer 40 ml ×1
9. Stop Solution 6 ml ×1
10. Biotined P24 Antibody 3 ml ×1
ASSAY PROCEDURE：
1. Add samples：Open the foil pouch and remove the microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control, 2 wells as P24 antigen control. Dispensing
75μl of samples or negative control or positive control or P24 antigen control to the respective wells. Then dispensing 25μl of biotined P24 antibody to each well except the blank well. Gently vibrating the microplate. Cover and incubate for 60 minutes at 37℃.
2. Wash the plate：Dilute 1 volume of wash buffer concentrate with 19 volumes of distilled water, mix well. Remove the solutions from all the wells. Fill the wells with the diluted wash solution ( 10~20 seconds to soak) then remove the diluted wash buffer from the wells. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
3. Add conjugate：Add the 100μl of conjugate to each well (except the blank well). Cover and incubate for 30 minutes at 37℃.
4. Wash the plate：Repeat the wash procedure as in step 2.
5. Add 50μl of substrate diluent and 50μl of TMB buffer to each well, mix well. Cover and incubate for 10 minutes at 37℃.
6. Stop reaction：Add 50μl stop solution to each well, mix well.
7. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.
RESULTS：
1. For the assay to be valid, the average OD value of negative controls must be less than or equal to 0.1 and the average OD value of positive controls must be greater than or equal to 0.8 and the average OD value of P24 antigen control must be greater than or equal to 0.8.
2. Cut off value = 0.10 + average OD value of negative control
3. OD value of the sample cut off value, it is positive for HIV 1+2 Ab / P24 Ag
OD value of the sample cut off value, it is negative for HIV 1+2 Ab / P24 Ag
PRECAUTIONS：
1. Allow all kit components to reach room temperature before use.
2. Follow the direction insert to control the reaction temperature and time strictly.
3. Do not mix components of different lot numbers to use.
4. The kit should be store at 2-8℃. Do not use kit components beyond their expiration date.
The HIV 1+2 Ab / P24 Ag EIA is a qualitative enzyme immunoassay for the in vitro detection of HIV infection, based on the detection of antibodies to HIV 1 and HIV 2 and the P24 antigen in human serum or plasma. This assay has the advantage of being sensitive,specific,simple,and fast. It has great significance in the diagnosis of HIV infection / screen for donated blood to prevent transmission of HIV to recipients and as an aid in clinical diagnosis of HIV-related infections.
REAGENTS： 96 tests
1. Microplate 96 wells
2. Negative Control 1 ml ×1
3. Positive Control 1 ml ×1
4. P24 Antigen Control 1 ml ×1
5. Conjugate 12 ml ×1
6. Substrate Diluent 6 ml ×1
7. TMB Buffer 6 ml ×1
8. 20×Wash Buffer 40 ml ×1
9. Stop Solution 6 ml ×1
10. Biotined P24 Antibody 3 ml ×1
ASSAY PROCEDURE：
1. Add samples：Open the foil pouch and remove the microplate. Set up 1 well as Blank, 2 wells as negative control, 2 wells as positive control, 2 wells as P24 antigen control. Dispensing
75μl of samples or negative control or positive control or P24 antigen control to the respective wells. Then dispensing 25μl of biotined P24 antibody to each well except the blank well. Gently vibrating the microplate. Cover and incubate for 60 minutes at 37℃.
2. Wash the plate：Dilute 1 volume of wash buffer concentrate with 19 volumes of distilled water, mix well. Remove the solutions from all the wells. Fill the wells with the diluted wash solution ( 10~20 seconds to soak) then remove the diluted wash buffer from the wells. Repeat the procedure for 5 times. Make sure that the rest volume is minimal, by tapping plate onto absorbent paper.
3. Add conjugate：Add the 100μl of conjugate to each well (except the blank well). Cover and incubate for 30 minutes at 37℃.
4. Wash the plate：Repeat the wash procedure as in step 2.
5. Add 50μl of substrate diluent and 50μl of TMB buffer to each well, mix well. Cover and incubate for 10 minutes at 37℃.
6. Stop reaction：Add 50μl stop solution to each well, mix well.
7. Read the absorbance at 450 nm. If a dual wavelength measurement is used, the reference wavelength should be selected from 620nm to 690nm.
RESULTS：
1. For the assay to be valid, the average OD value of negative controls must be less than or equal to 0.1 and the average OD value of positive controls must be greater than or equal to 0.8 and the average OD value of P24 antigen control must be greater than or equal to 0.8.
2. Cut off value = 0.10 + average OD value of negative control
3. OD value of the sample cut off value, it is positive for HIV 1+2 Ab / P24 Ag
OD value of the sample cut off value, it is negative for HIV 1+2 Ab / P24 Ag
PRECAUTIONS：
1. Allow all kit components to reach room temperature before use.
2. Follow the direction insert to control the reaction temperature and time strictly.
3. Do not mix components of different lot numbers to use.
4. The kit should be store at 2-8℃. Do not use kit components beyond their expiration date.
公司简介
Beijing Jinsheng Lida Bio-Tech Co. Ltd (BJLB) is a professional kits (ELISA/ Rapid Diagnostic) and medical instrument provider, and engages in offering products with dependable quality and excellent performance to all over the world.
BJLB possesses a well trained sale team to ensure to provide 24 hour high quality service; BJLB possesses professional technical consultants, and can duly resolve any problems during the use; Before sent to clients, our products are monitored rigidly during the whole course, so that the quality thereof can be assured.
BJLB regards “create one grade quality and service, contribute to the mankind health” as the management policy to serve society and public.
BJLB possesses a well trained sale team to ensure to provide 24 hour high quality service; BJLB possesses professional technical consultants, and can duly resolve any problems during the use; Before sent to clients, our products are monitored rigidly during the whole course, so that the quality thereof can be assured.
BJLB regards “create one grade quality and service, contribute to the mankind health” as the management policy to serve society and public.
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