Mouse mAb to CD18 LFA-1,integrin
Clone 68-5A5
lsotype Mouse IgG2a
Source
A BALB/c mouse was immunized with stimulated human leucocytes. Splenocytes were fused with mouse myeloma NS 1 cells.
Specifications
The mAb 68-5A5 reacts with human CD18, a 95 kD glycoprotein non-covalently linked to CD11a, b and c molecules. MW of the heterodimer in SDS gels is 180 kD. The protein is expressed on leucocytes and functions as receptor for ICAM-1 (CD54) and is important
Applications
The mAb has been applied in flow cytometry and can be used for blocking cell-cell interactions.
Frozen section +
Paraffin sections —
Flow cytometry +
ELISA N.D.
Blocking
Immuno-precipitation N.D.
Western blotting N.D.
References
McMichael A.J.et al., Leucocyte typing Ill, Oxford University Press, Oxford, 1987.