酸性磷酸酶
性状 | lyophilized powder |
保存温度 | −20°C |
Biochem/physiol Actions
Acid phosphatases (APase) are a family of enzymes that non-specifically catalyze the hydrolysis of monoesters and anhydrides of phosphoric acid to produce inorganic phosphate at an optimum pH of 4 to 7.
APase is assayed spectrophotometrically in a 1.1 ml reaction mixture containing 41 mM citrate buffer, pH 4.8 at 37 °C, 6.9 mM p-nitrophenyl phosphate, and 0.015–0.025 unit APase.APase is soluble in cold water (0.15–0.25 unit/ml). Prepare solution immediately before use.
Storage/Stability
Store the product at –20 °C. W hen stored at –20 °C, the enzyme retains activity for at least one year.APase can lose half its activity after 30 minutes at 60 °C and 100% of its activity after two hours at 70 °C.