TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)
TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+)
Catalog # 80578
Size: 96 reactions
DESCRIPTION: The TNKS2 Histone Ribosylation Assay Kit (Biotin-labeled NAD+) is
designed to measure Tankyrase 2 (TNKS2) activity for screening and profiling
applications. TNKS2 catalyzes the NAD-dependent addition of poly(ADP-ribose) to the
substrate proteins. The TNKS2 assay kit comes in a convenient 96-well format, with
purified TNKS2 enzyme, histone mixture, and PARP assay buffer for 100 enzyme
reactions. The key to the TNKS2 Histone Ribosylation Assay is the biotinylated NAD+
substrate. With this kit, only three simple steps are required for TNKS2 reactions. First,
histone proteins are coated on a 96-well plate. Next, the biotinylated NAD+ substrate is
incubated with an assay buffer that contains the TNKS2 enzyme. Finally, the plate is
treated with streptavidin-HRP followed by addition of the HRP substrate to produce
chemiluminescence that can be measured using a chemiluminescence reader.
COMPONENTS:
APPLICATIONS: Great for studying enzyme kinetics and screening small molecular
inhibitors for drug discovery and HTS applications.
STABILITY: Up to 1 year when stored as recommended.
REFERENCE: Brown, J.A., Marala, R.B. J. Pharmacol. Toxicol. Methods 2002 47:137-41.
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