PDE1B TR-FRET Assay Kit

PDE1B TR-FRET Assay Kit

Catalog # 60704
Size: 96 reactions

DESCRIPTION: Phosphodiesterases (PDEs) play an important role in dynamic
regulation of cAMP and cGMP signaling. PDE1B is a calcium/calmodulin-dependent
cyclic nucleotide phosphodiesterase that is highly expressed in the striatum. It plays a
physiological role in the central nervous system, and PDE1B activity has been linked to
impaired cognition and spatial learning.

The PDE1B TR-FRET Assay Kit is designed for identification of inhibitors of PDE1B
using TR-FRET (Time Resolved Fluorescence Resonance Energy Transfer) technology.
The assay is based on the generation of FAM-labeled nucleotide monophosphates by
the PDE1B. These phosphate groups bind to terbium-labeled nanoparticles, resulting in
energy transfer from the terbium to the FAM, which emits a fluorescent signal at 520 nm.
The change in fluorescent intensity can be easily measured using a fluorescence plate
reader.

The PDE1B TR-FRET Assay Kit comes in a convenient 96-well format, with purified
PDE1B enzyme, fluorescently labeled PDE1B substrate (cAMP), binding agent, and
PDE assay buffer for 100 enzyme reactions. Using this kit, only two simple steps on a
microtiter plate are required for the PDE1B activity assay. First, the fluorescent-labeled
cAMP is incubated with a sample containing PDE1B for 1 hour. Second, a binding agent
and a terbium donor are added to the reaction mix and incubated for 30 minutes. Then,
fluorescence intensity can be measured using a fluorescence reader.

COMPONENTS:

APPLICATIONS: Great for screening small molecular inhibitors for drug discovery and
HTS applications.

STABILITY: At least 6 months from date of receipt when stored as directed.

REFERENCE(S): Siuciak JA, et al. (2007) Neuropharmacology 53(1):113-24.

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