L3MBTL1 Inhibitor Screening Assay Kit
L3MBTL1 Inhibitor Screening Assay Kit
Catalog # 55100
Size: 384 reactions
DESCRIPTION: The L3MBTL1 Inhibitor Screening Assay Kit is designed to measure the
inhibition of L3MBTL1 binding to its substrate. The kit comes in a convenient AlphaLISA®
format, with enough biotinylated histone peptide substrate, assay buffer, detection buffer
and purified GST-tagged L3MBTL1 MBT domain to perform a total of 384 enzyme
reactions. The key to the kit is the specific binding of the L3MBTL1 MBT domain to the
methylated-peptide substrate. With this kit, only three simple steps on a microtiter plate
are required. First, a sample containing L3MBTL1 and an inhibitor of choice is incubated
with the biotinylated substrate for thirty minutes. Next, acceptor beads are added, then
donor beads, followed by reading the Alpha-counts.
COMPONENTS:
APPLICATIONS: Useful for the study of MBT domain binding assays, screening
inhibitors, and selectivity profiling.
STABILITY: At least one year from date of receipt when stored as directed.
REFERENCE: Min, J., et al., Nat Struct Mol Biol. 2008 Jan;15(1):114.
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