HemH | protoporphyrin ferrochelatase (cyanobacterial)
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| application information | ||
| recommended dilution | 1: 3000 with standard ECL, West pico (WB) | |
| expected | apparent MW | 46 kDa | |
| confirmed reactivity | Synechocystis sp. PCC 6803 | |
| predicted reactivity | cyanobacteria | |
| not reactive in | no confirmed exceptions from predicted reactivity known in the moment | |
| additional information | loads higher than 0.5 µg of chlorophyll per well are not recommended | |
| selected references | to be added when available | |
application example Membrane fraction (0.5 µg chl), prepared from Synechocystis sp. PCC 6803, was separated on 12% SDS-PAGE and blotted in 2 hours at RT to PVDF membrane. Membrane was blocked for 1h using 0.2% tween 20 in TBS, probed with anti-PsbI (1:10 000) antibody in TTBS overnight at 8ºC and secondary anti-rabbit in TTBS (1:10000,1h, room temperature). Blot was developed for 5 min in West pico substrate and image of the blot was obtained using Fuji LAS 4000. Courtesy Dr. Roman Sobotka, Laboratory of Photosynthesis, Trebon,Czech Republic | ![]() |
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