JIM7抗体，JIM7 antibody
Jim7抗同型半乳糖醛酸聚糖
单克隆抗体抗同型半乳糖醛酸聚糖/ JIM7
（鼠型IgA，JIM7）
特异性：该抗体识别果胶多糖的同型半乳糖醛酸聚糖区域。据了解它与其他聚合物没有交叉反应。能识别若干物种中的果胶多糖。抗体能识别同型半乳糖醛酸聚糖的部分甲酯化的表位，但不与非酯化抗同型半乳糖醛酸聚糖结合。
| Antibody Name: | JIM7 |
| Related Experiments: | Arabidopsis inflorescence stem expansion glycoprofiling |
| Catolog#: | PP-007 |
| Isotype: | IgA |
| Antibody Type: | Monoclonal |
| Source Animal: | Rat |
| Detail Info for Purified Antibody: | |
| Immunogen: | Protoplasts prepared from suspension-cultured cells |
| Antigen: | Homogalacturonan (partially methyl-esterified) |
| Plant Origin: | Carrot (Daucus carota) |
| Working Concentration: | |
| Status of Antibody: | Hybridoma supernatant |
| Polysaccharide Cross Reactivity: | JIM7.pdf |
| Epitope Structure for Carbonhydrate Antigen: | GalA1->4MeGalA1->4MeGalA1->4MeGalA1->4MeGalA1->4GalA |
| M.W. of Protein Antigen(KDa): | |
| Protein Cross Reactivity: | |
| References: | J.P. Knox, P.J. Linstead, J. King, C. Cooper, and K. Roberts. (1990) Pectin esterification is spatially regulated both within cell walls and between developing tissues of root apices. Planta 181:512-521.
W.G.T. Willats, G. Limberg, H.C. Buchholt, G.-J. Van Alebeek, J. Benen, T.M.I.E. Christensen, J. Visser, A. Voragen, J.D. Mikkelsen, and J.P. Knox. (2000) Analysis of pectic epitopes recognised by hybridoma and phage display monoclonal antibodies using defined oligosaccharides, polysaccharides, and enzymatic degradation. Carbohydr.Res.327:309-320, 2000. M.H. Clausen, W.G.T. Willats, and J.P. Knox. (2003) Synthetic methyl hexagalacturonate hapten inhibitors of anti-homogalacturonan monoclonal antibodies LM7, JIM5 and JIM7. Carbohydr.Res. 338:1797-1800, 2003. |
| Comments: |
JIM7 binds to a relatively heavily methylesterified epitope found in cell walls of diverse species. The epitope structure shown is the most efficient competitor identified. Other competitors that are almost equally effective are: GalA1->4GalA1->4MeGalA1->4MeGalA1->4MeGalA1->4GalA and MeGalA1->4GalA1->4MeGalA1->4GalA1->4MeGalA1->4GalA. |
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| Experiment Title:Arabidopsis inflorescence stem expansion glycoprofiling | |
|---|---|
| Experiment Title: | Arabidopsis inflorescence stem expansion glycoprofiling |
| Description: |
Series of transverse sections at various regions of a bolting inflorescenc e primary stem to capture compositional changes during cell wall development. |
| Organism: | Arabidopsis thaliana |
| Tissues: | Inflorescence stem |
| Treatments: | Developmental stage (young, maximum growth rate, and cessation) |
| Design: | Glycoprofiling_Arab_inflr_exp-design.pdf |
| Antibodies Employed: | MAC207 CCRC-M1 CCRC-M2 CCRC-M7 CCRC-M8 CCRC-M9 CCRC-M11 CCRC-M12 CCRC-M13 CCRC-M14 CCRC-M15 CCRC-M16 CCRC-M22 CCRC-M30 CCRC-M31 CCRC-M32 CCRC-M34 CCRC-M36 CCRC-M38 CCRC-M44 CCRC-M48 CCRC-M49 CCRC-M70 JIM1 JIM3 JIM4 JIM5 JIM7 JIM8 JIM11 JIM12 JIM13 JIM14 JIM15 JIM16 JIM17 JIM19 JIM20 JIM84 JIM93 JIM94 JIM101 JIM131 JIM132 JIM133 JIM136 JIM137 LM10 LM18 LM19 LM20 MAC204 MAC265 MAC266 PN 16.4B4 |
| Immunolableing Protocol: | Immunolabeling_protocol_for_CCRC.txt |
| Imageing Protocol: | Imaging_protocol_for_CCRC.txt |
| Contributor Name: | Hardy Hall, Jingling Cheung, Brian Ellis |
| Contributor Address: | Michael Smith Laboratories, University of British Columbia, Vancouver, BC V6T 1Z4, Canada |
| Related Publication: | Immunoprofiling reveals unique cell-specific patterns of wall epitopes in the expanding Arabidopsis stem (DOI: 10.1111/tpj.12111) |
| Publication Status: | In Press |
| Related Image Sets: | CCRC-M1-Panel CCRC-M11-panel CCRC-M12-panel CCRC-M13-panel CCRC-M14-panel CCRC-M15-panel CCRC-M16-panel CCRC-M2-panel CCRC-M22-panel CCRC-M30-panel CCRC-M31-panel CCRC-M32-panel CCRC-M34-panel CCRC-M36-panel CCRC-M38-panel CCRC-M44-panel CCRC-M48-panel CCRC-M49-panel CCRC-M7-panel CCRC-M70-panel CCRC-M8-panel CCRC-M9-panel JIM1-panel JIM101-panel JIM11-panel JIM12-panel JIM13-panel JIM131-panel JIM132-panel JIM133-panel JIM136-panel JIM137-panel JIM14-panel JIM15-panel JIM16-panel JIM17-panel JIM19-panel JIM20-panel JIM3-panel JIM4-panel JIM5-panel JIM7-panel JIM8-panel JIM84-panel JIM93-panel JIM94-panel LM10-panel LM18-panel LM19-panel LM20-panel MAC204-panel MAC207-panel MAC265-panel MAC266-panel PN 16.4B4-panel |
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