抗体

微管蛋白γ单克隆抗体

基本信息
产品名称:
微管蛋白γ单克隆抗体
英文名称:
Gamma Tubulin
国产/进口:
国产
产地/品牌:
雅吉生物
型号:
33313M
参考报价:
总点击数:
629
更新日期:
2026-03-26
产品类别:

性能参数
 英文名称Gamma Tubulin
中文名称微管蛋白γ单克隆抗体
别    名Centrosome Marker; Gamma 1 tubulin; gamma tubulin; gamma-tubulin; Gamma 2 tubulin; Gamma Tubulin 1; Gamma Tubulin 2; Gamma tubulin complex component 1; GCP 1; GCP-1; GCP1; MGC131994; TUBG; TUBG1; TUBG2; Tubulin gamma 1 chain; Tubulin gamma 2 chain; Xgam; TBG1_HUMAN; TBG2_HUMAN; Tubulin gamma-1 chain; Gamma-1-tubulin; Gamma-tubulin complex component 1.  
研究领域细胞生物  神经生物学  细胞骨架  
抗体来源Mouse
克隆类型Monoclonal
克 隆 号8D11
交叉反应Human, Mouse,  (predicted: Rat, )
产品应用WB=1:500-5000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 (石蜡切片需做抗原修复)
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
分 子 量50kDa
细胞定位细胞浆 
性    状Liquid
浓    度1mg/ml
免 疫 原KLH conjugated synthetic peptide derived from human Gamma Tubulin: 
亚    型IgG
纯化方法affinity purified by Protein G
储 存 液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
PubMedPubMed
产品介绍Centrosome Marker
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end.
The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.
 
Function:
Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome. Pericentriolar matrix component that regulates alpha/beta tubulin minus-end nucleation, centrosome duplication and spindle formation.
 
Subunit:
Interacts with TUBGCP2 and TUBGCP3. Interacts with B9D2 (By similarity). Interacts with CDK5RAP2; the interaction is leading to centrosomal localization of TUBG1 and CDK5RAP2. Interacts with PIFO.
 
Subcellular Location:
Cytoplasm, cytoskeleton, centrosome.
 
Tissue Specificity:
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
 
Post-translational modifications:
Phosphorylation at Ser-131 by BRSK1 regulates centrosome duplication, possibly by mediating relocation of gamma-tubulin and its associated proteins from the cytoplasm to the centrosome.
 
Similarity:
Belongs to the tubulin family.
 
SWISS:
P23258
 
Gene ID:
7283
 
Database links:
Entrez Gene: 27175 Human
 
Entrez Gene: 7283 Human
 
Entrez Gene: 103733 Mouse
 
Entrez Gene: 103768 Mouse
 
Entrez Gene: 252921 Rat
 
Entrez Gene: 680991 Rat
 
Omim: 191135 Human
 
Omim: 605785 Human
 
SwissProt: P23258 Human
 
SwissProt: Q9NRH3 Human
 
SwissProt: P83887 Mouse
 
SwissProt: Q8VCK3 Mouse
 
SwissProt: P83888 Rat
 
Unigene: 279669 Human
 
Unigene: 708059 Human
 
Unigene: 142348 Mouse
 
Unigene: 479145 Mouse
 
Unigene: 154431 Rat
 
 
 
Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
 
结构蛋白(Structural Proteins)
gamma tubulin 微管蛋白是存于中心体的另一种微管蛋白, gamma微管蛋白对微管的形成具有重要作用。gamma微管蛋白通过与β-微管蛋白的相互作用帮助微管的成核反应(nucleation)。即在微管的组装中gamma微管蛋白先形成一个圆或形成钩环结构, γ微管蛋白的这种结构可指导微管蛋白二聚体结合上去并进行微管的组装。gamma微管蛋白在微管的组装中起关键作用。
产品图片
Sample:
A549(Human) Cell Lysate at 30 ug
MCF-7(Human) Cell Lysate at 30 ug
U87MG(Human) Cell Lysate at 30 ug
Primary: Anti- Gamma Tubulin (bsm-33313M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (bsm-33313M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Gamma Tubulin) Polyclonal Antibody, Unconjugated (bsm-33313M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
公司简介
       上海雅吉生物科技有限公司成立于2011年3月,是一家面向生命科学领域,科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学,通过公司各个部门所有员工的共同努力在行业内拥有较高知名度,深得新老客户的厚爱,本着“优质、服务、信誉”的精神,坚持以先进的技术、优质的产品、良好的信誉,为国内外广大用户提供优质生物产品和服务。公司在重视产品质量的同时,也建立了一套集技术支持、物流、售后服务等多个部门联动服务体系,努力把我们方便、快捷、周到的服务提供给每一个客户本研究所郑重承诺:质量保证、供货及时、服务周到,公司总投资超过2000万元,并先后引进了自动化设备,组建了专业的研发技术团队。凭借着洁净化的生产车间、标准化的质量管理系统,为生产高质量的产品提供了有效保证。
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试剂盒售后:本公司出售的试剂盒均保质保量,质量问题均可免费退换,另提供免费代测服务。 细胞售后: 1. 细胞运输丢失、瓶身破损、培养液严重漏液等,重发; 3. 细胞收到当天以及第2,3天请拍照,未告知的视为产品合格。4-10天内出现问题,请提供细胞照片和细胞出现问题的照片以及细胞相关操作的详细步骤,并跟我公司人员及时沟通判定是否重发,具体可以参照我官网或者随货说明书相关售后条款。
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