Qiagen 74104 RNA小量提取试剂盒(50)
名称 | 品牌 | 货号 | 规格 | 保存 |
RNA小量提取试剂盒(50) RNeasy Mini Kit (50) |
Qiagen | 74104 | 50次 | RT |
Source | Starting material | Average yield | ||||||||
Animal cells | Micro | Mini/ QIAwave Mini |
Midi | Maxi | 96-well | Micro | Mini/ QIAwave Mini |
Midi | Maxi | 96-well |
LMH | 5x105 | 1x106 | 7x107 | 5x108 | 1x105 | 6µg | 12µg | 850µg | 5.7mg | 1.3µg* |
HeLa | 5x105 | 1x106 | 7x107 | 4x108 | 1x105 | 5µg | 15µg | 1000µg | 6.0mg | 1.6µg* |
COS-7 | 5x105 | 1x106 | 3x107 | 1.8x108 | 1x105 | 17.5µg | 35µg | 950µg | 5.8mg | 3.1µg* |
Lymphocytes (unstimulated) |
5x105 | 1x106 | 1x108 | 5x108 | – | – | 0.5µg | 50µg | 0.3mg | – |
Huh7 | 5x105 | – | – | – | 1x105 | 7.5µg | – | – | – | 2.0µg* |
Jurkat | 5x105 | – | – | – | 1x105 | – | – | – | – | 1.4µg* |
Mouse tissue | ||||||||||
Liver | 5mg | 10mg | 200mg | 1g | – | 15µg | 40µg | 700µg | 3.6mg | – |
Lung | 5mg | 10mg | 100mg | 0.5g | – | 5µg | 10µg | 130µg | 0.6mg | – |
Spleen | 5mg | 10mg | 200mg | 1g | – | 15µg | 35µg | 600µg | 3.2mg | – |
Yeast cells | ||||||||||
S. cerevisiae | – | 1 x 107 | 2x108 | 1x109 | – | – | 25µg | 450µg | 2.4mg | – |
RNeasy Mini Kit | (50) |
Catalog no. | 74104 |
Number of preps | 50 |
RNeasy Mini Spin Columns (pink) | 50 |
Collection Tubes (1.5 ml) | 50 |
Collection Tubes (2 ml)* | 50 |
Buffer RLT*† | 45 ml |
Buffer RW1† | 45 ml |
Buffer RPE† (concentrate) | 11 ml |
RNAse-Free Water | 10 ml |
Quick-Start Protocol | 2 |
问题 | 可能原因 | 解决方案 |
RNA产量低 | 样本裂解不充分; 洗脱体积过大; 离心柱堵塞 |
确保样本彻底裂解(如组织研磨充分); 减少洗脱体积(如使用30μl); 离心前确保裂解液无沉淀 |
RNA纯度低 (OD260/OD280<1.8) |
蛋白质残留; Buffer RPE洗涤不充分 |
增加Buffer RW1洗涤次数; 确保Buffer RPE按要求加入乙醇并充分洗涤; 避免样本过量 |
RNA降解 | 样本处理时间过长; RNase污染 |
使用新鲜样本并快速处理; 严格无RNase操作; 确保Buffer RLT充分裂解并失活RNase |
单位名称: |
详细地址:
北京市海淀区马连洼北路九号东居兴业写字楼
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qq:
523539965
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联系电话: |
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