牛血清白蛋白,BSA,A3311
Synonym: BSA, Bovine albumin
CAS Number: 9048-46-8
EC Number: 232-936-2
MDL number: MFCD00130384
Description
Preparation Note Serum albumin may be referred to as Cohn Fraction V. This naming convention is taken from the original Cohn1 method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Cohn Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Application This albumin has been qualified for use in media designed to support in vitro applications such as mouse blastocyst and morulae culture.
Biochem/physiol Actions Bovine serum albumin is broadly used as an additive to cell culture media, especially serum-free media. It provides a range of benefits including protection from oxidative damage and stabilization of other media components such as fatty acids and pyridoxal.
Protocols & Applications Select the right albumin for your application with the Albumin Selection Guide
Properties
product line BioXtra
assay ≥96% (agarose gel electrophoresis)
form powder
mol wt mol wt ~66 kDa
suitability suitable for mouse embryo cell culture
storage temp. 2-8°C
Safety
Personal Protective Equipment Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
WGK Germany 3
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