化学发光试剂盒

普通化学发光检测试剂盒

       KC™化学发光试剂盒使用Chemiluminescent系统进行Western Blotting实验的蛋白检测，灵敏度高，背景非常低。其底物是一种高灵敏度增强型底物，用于检测免疫印迹中的辣根过氧化物酶（Horseradish peroxidase，HRP），这种底物能产生强烈的信号输出，可检测到皮克（pg）级的抗原。其信号灵敏度高、强度高、持续时间长，方便用胶片或者CCD成象仪进行检测。检测灵敏度超过ECL底物5倍，如果如果一级抗体用ECL底物1:100稀释过，然后再用Chemiluminescent底物进行1:500稀释，仍然可以在胶片上重复多次曝光以得到最佳结果。

General Western Blot Detection Kit was designed to make western detection of proteins easier by providing the highest level of sensitivity with least amount of background in a chemiluminescent reagent system. The substrate provided is a highly sensitive enhanced substrate for detecting horseradish peroxidase (HRP) on immunoblots. This substrate’s extremely intense signal output enables detection of picogram amounts of antigen. The sensitivity, intensity and duration of the signal allow for easy detection of HRP using CCD imagers as well as with films. The sensitivity increase over ECL Substrate can be up to 5 fold. If you have been using the primary antibody at 1:100 dilutions with ECL substrate, then use a 1:500 dilution with our Chemiluminescent Substrate. Blots can also be repeatedly exposed to film to obtain optimal results.

General Western Blot Detection Reagents