Lumazone 系列是美国Princeton Instruments(Roper Technologies, Inc. 集团子公司)研制的活体生物发光成像系统,可以观察并成像将生物发光标记后的植物整体标本,并结合专业的分析软件完成植物体内基因表达的原位跟踪、植物突变体监测和筛选以及单细胞内的基因表达等研究。
Roper 公司一直致力于将其最佳性能的光学成像系统推荐给客户,并凭借在生命科学研究领域多年来积累的经验,能够根据客户需求为客户量身定制成像系统,已成为在此领域中具领先地位的系统供应商之一。
为了满足植物学研究的需要,解决植物活体荧光成像时叶绿体荧光干扰的问题,博益公司推出了多款专业型的植物整体分子影像系统,包括最新的 Lumazone SOPHIA 2048B、Lumazone PyLoN1300B 和 Lumazone PyLoN2048B 等,并针对不同的实验需求配置突变体筛选暗箱或光周期暗箱。实验无需将植物样本做任何处理,并可通过生物发光(LUC)和荧光方法实时观察植物样本基因表达的变化,准确定量分析。
Lumazone PyLoN 2048B特点:
1.植物活体光学成像的最佳选择
2.活体检测最高灵敏度,最大成像视野
3.背景噪声最低
4.实现长时间曝光
» CCD 温度:-120℃
» 暗电流:0.3e-/p/h @-120。C
» 读出噪音:3e-rms@50KHz 读出速度
» 芯片尺寸:27.6 x 27.6mm
» 量子效率:>90% @500-700nm;>50%@400-500nm 或700-880nm
常见应用:高通量,弱表达、突变体筛选,逆境处理研究,生物节律,激素信号,蛋白质互作,甲基化
植物学部分参考文献:
- Arabidopsis thaliana mutant lpsi reveals impairment in the root responses to local phosphate availability. Plant Physiology and Biochemistry (2014) 77: 60-72
- A Pre-mRNA-Splicing Factor Is Required for RNA-Directed DNA Methylation in Arabidopsis. PLoS Genet (2013) 9(9): e1003779
- Aequorin-Based Luminescence Imaging Reveals Stimulus- and Tissue-Specific Ca2+ Dynamics in Arabidopsis Plants. Molecular Plant (2013) 6(2): 444-455
- Singlet oxygen scavenging activity of tocopherol and plastochromanol in Arabidopsis thaliana: relevance to photooxidative stress. Plant, Cell & Environment (2013) 37(2): 392-401
- Towards the two-dimensional imaging of spontaneous ultra-weak photon emission from microbial, plant and animal cells. Nature, Scientific Reports (2013) 3:1211
- Generation of a luciferase-based reporter for CHH and CG DNA methylation in Arabidopsis thaliana. Silence (2013), 4:1
- Ultra-weak photon emission as a non-invasive tool for the measurement of oxidative stress induced by UVA radiation inArabidopsis thaliana. Journal of Photochemistry and Photobiology B (2013) 123: 59-64
- A histone acetyltransferase regulates active DNA demethylation in Arabidopsis. Science (2012) 336:1445-1448
- A DNA 3’-phosphatase functions in active DNA demethylation in Arabidopsis. Mol Cell (2012) 45(3): 357-370
- Antisilencing role of the RNA-directed DNA methylation pathway and a histone acetyltransferase in Arabidopsis. Proc. Natl. Acad. Sci. (2012) 109: 11425-11430
- A Plasma Membrane Receptor Kinase, GHR1, Mediates ABA- and H2O2-regulated Stomatal Movement in Arabidopsis. Plant Cell (2012) 24: 2546-2561
- Development of a luciferase-based reporter of transcriptional gene silencing that enables bidirectional mutant screening in Arabidopsis. Silence (2012) 3(1):6
- The Arabidopsis AP2/ERF Transcription Factor RAP2.11 Modulates PlantResponse to Low-Potassium Conditions. Mol Plant, Mar (2012) 10: 1093/mp/sss003
- LIGHT-REGULATED WD1 and PSEUDO-RESPONSE REGULATOR9 Form a Positive Feedback Regulatory Loop in the Arabidopsis Circadian Clock. The Plant Cell (2011) 23: 486-498
- The Plant Cuticle Is Required for Osmotic Stress Regulation of Abscisic Acid Biosynthesis and Osmotic Stress Tolerance in Arabidopsis. The Plant Cell (2011) 23: 1971-1984
- A Peroxidase Contributes to ROS Production during Arabidopsis Root Response to Potassium Deficiency. Molecular Plant (2010) 3:420-427
- Ethylene Mediates Response and Tolerance to Potassium Deprivation in Arabidopsis. The Plant Cell (2009) 21: 607-621
- NRPD4, a protein related to the RPB4 subunit of RNA polymerase II, is a component of RNA polymerases IV and V and is required for RNA-directed DNA methylation. Genes & Development (2009) 23:318-330
- A conserved transcriptional regulator is required for RNA-directed DNA methylation and plant development. Genes & Development (2009) 23: 2717-2722
- Disruption of Arabidopsis CHY1 Reveals an Important Role of Metabolic Status in Plant Cold Stress Signaling. Molecular Plant (2009) 2:59-72
- Isolation and Characterization of cul1-7, a Recessive Allele of CULLIN1 That Disrupts SCF Function at the C Terminus of CUL1 in Arabidopsis thaliana. Genetics (2009)181: 945-963
- COP1-Mediated Ubiquitination of CONSTANS Is Implicated in Cryptochrome Regulation of Flowering in Arabidopsis. The Plant Cell (2008) 20: 292-306
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