Boyden 细胞趋化小室
用于细胞趋化,详见麒麟医用仪器厂主页进入下载页面下载相关资料。
微孔小室中的趋化实验:
微孔小室中的趋化实验是根据靶细胞(单核巨噬细胞,中性粒细胞或淋巴细胞等)能够趋化性主动迁移,穿过一定孔径的滤膜而设计的。滤膜将小室分隔成上下两部分。靶细胞在上面,趋化因子在下面,趋化因子通过滤膜形成梯度,细胞则沿着梯度穿过膜孔,附在膜的下面,染色并计数滤膜下表面的细胞数即可测出趋化因子的趋化能力。
趋化材料和趋化时间的选择:
趋化滤膜的材料和孔径需根据靶细胞的大小选择:中性粒细胞用3um孔径的PVPF聚碳酸膜,趋化时间为30分钟;单核细胞用8um孔径的PVPF聚碳酸膜,趋化时间为90分钟;粘附力弱的淋巴细胞则下表面复以明胶或纤粘素的5um或8umPVPF聚碳酸膜,以免淋巴细胞穿过膜后落入下室,趋化时间为180分钟.
(海门市麒麟医用仪器厂 )
www.qilinyq.com.cn
Hm-Kylin
公司简介:
海门市麒麟医用仪器厂是生产实验室仪器(摇床、离心机、混合器、紫外仪等)及实验用耗材(硅胶塞、吸头、离心管、梅标板等)的专业工厂,82年建厂至今,依靠精湛的生产工艺和先进的管理理念,在国内科研领域享有较高的信誉。长期以来,我厂本着“以人为本,服务科研”的最高服务理念,与各高等学府、科研院所紧密合作,树立了麒麟医用仪器的良好企业形象。
1990年,我厂产品被第11届北京亚运会兴奋剂检测中心选为专用检测仪器。
2008年,我厂产品再次被第29届北京奥运会兴奋剂检测中心选为专用检测仪器。
“在创新中追求卓越,在发展中中服务科研”,已成为高科技条件下麒麟人的新誓言。
Haimen City Qilin Medical Instrument Factory,founded in 1982,specializes in the manufacturing of lab instrument and consumable. With its perfect manufacturing skill and advanced managing experience,our factory has gained high credit standing in the field of scientific research nationwide. Our tiptop service concept is human oriented while serving the scientific research. We have tight cooperation with a large number of universities and scientific research institutes, during the course of which, we have built up a sound enterprise image.
In the year of 1990,our product were selected as the special detecting instrument by the 11th Peking Asian Games Stimulant Test Center.
In the year of 2008,our product were selected again as the special detecting instrument by the 29th Beijing Olympic Games Stimulant Test Center.
In the circumstance of high technology,we have a brand new oath:innovating while pursuing excellence;developing while serving scientific research.
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江苏省海门市开发区定海路118号
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