Recombinant Chymotrypsin
Recombinant Human Chymotrypsin
Catalog Number:RCT10
CAS: 9004-07-3
EC: 3.4.21.1
Storage temperature: 2-8℃
Source: Recombinant huamn chymotrypsin is a genetically engineered protein expressed in E.coli
Protein Sequence:The amino acid sequence of recombinant human chymotrypsin is identical to human chymotrypsin
1.DESCRIPTION
Chymotrypsin is a recombinant serine endopeptidase expressed in E.coli, the gene sequence is the same as human chymotrypsin. Recombinant chymotrypsin hydrolyzes at the carboxyl side of aromatic amino acids residues: Tyr, Phe and Trp. Cleavage may also be observed, but at a lower rate, at Leu and Met. Chymotrypsin activity is optimal in pH 7.0–9.0.
2.MAIN FEATURES
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Source |
Recombinant E. coli |
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Appearance |
White or off white powder |
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Specific activity |
≥1000 USP units/mg pro. |
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SDS-PAGE |
Single major band |
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SDS-PAGE (MW) |
24.0±2.4 kDa |
UNIT DEFINITION: One unit will hydrolyze 1.0 μmol of N-alpha-acetyl-L-tyrosine ethyl ester(ATEE) per min at pH 7.0 at 25 °C.
3.RECOMMEND USAGE
Prepare 1-10mg/ml recombinant human chymotrypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
4.STABILITY OF STORAGE AND TRANSPORT
（1）Stability of storage: Recombinant human chymotrypsin lyophilized should be stored under 2-8℃ in sealed container.
It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. Avoid repeated freezing and thawing.
（2）Stability of transport: The product is stable by blue ice insulation transport.
5.PRODUCT ADVANTAGE
- Animal origin free: Recombinant human chymotrypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
- Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
- High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
- Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
- Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015quality system and GMP guidelines.
- Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.
6.PRODUCT USAGE
Used alone, or with other proteases to interact with digestive proteins, or other special uses such as organic synthesis.
7.RELATED PRODUCTS
Recombinant carboxypeptidase B
Sequencing grade chymotrypsin
Recombinant trypsin
Recombinant enterokinase
上海雅心生物技术有限公司主要从事基因工程蛋白酶的研发和生产，目前的主要品种为基因工程羧肽酶B和基因工程胰蛋白酶；为本公司自主研发，拥有自主知识产权。公司拥有自组研发团队，拥有世界先进的重组生物技术；自主研发的重组生物用酶为世界上生物用酶的首创！基因工程羧肽酶B和基因工程胰蛋白酶是由基因工程方法生产，纯度高，不含杂酶，无羧肽酶A、糜蛋白酶等其他蛋白酶活性；无动物源性，出口产品无需进行终产品的动物源性检验。制品中不含蛋白酶抑制剂，产品安全。 公司秉承“科技致胜”的理念，坚持“提供高纯度重组蛋白酶”，并愿与广大同行交流，有需求的客户提供优质的服务！ 产品经多家医药公司检验和试用，认为符合重组人胰岛素生产的要求（5L和50L重组人胰岛素的生产），在纯度上高于sigma公司的产品（HPLC法）；在比活上与sigma公司的产品类似。
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详细地址：
上海市徐汇区银都路466号1号楼101室
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492549087
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联系电话：
021-54336592
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