Carboxypeptidase B 羧肽酶B
Recombinant Carboxypeptidase B
Catalog Number:RCPB01
CAS: 9025-24-5
EC: 3.4.17.2
Source: Expressed in E. Coli.
DESCRIPTION
Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from the C-terminal end of polypeptides. The optimum pH is 8.0, and pI is 6.0. Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.
ADVANTAGES
Animal origin free:YaxinBio recombinant carboxypeptidase B is animal components free, eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B.
Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage.
High purity:
1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating proteases activities found in extracted enzymes with lower purity level.
2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A.
3) Less than 10ppm of recombinant trypsin.
MAIN FEATURES
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Source |
E. Coli |
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Format |
White or white-like or yellowish lyophilized |
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Additives |
Tris salts, NaCl salts and carbohydrates |
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Protein content |
35% ～ 60% |
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Specific activity |
≥170 units/mg pro. |
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Purity |
Single main band on SDS-PAGE |
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M.W. |
33.8kD±3.4kD |
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Contaminant activity |
Less than 10 ppm of recombinant trypsin. |
UNIT DEFINITION：One Unit of carboxypeptidase B activity hydrolyzes one micromole of hippuryl-L-arginine per minute at 25℃, pH 7.65.
RECOMMEND USAGE
Prepare 1-10mg/ml carboxypeptidase B with sterile water or 25mM Tris-HCl pH 7.65. The ratio to aimed protein is 1:50 to 1:1000 (w/w), the optimum pH is pH 7.0-9.0.
STORAGE INSTRUCTIONS
Recommend recombinant carboxypeptidase B lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃, It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
E-mail：sales@yaxinbio.com
上海雅心生物技术有限公司主要从事基因工程蛋白酶的研发和生产，目前的主要品种为基因工程羧肽酶B和基因工程胰蛋白酶；为本公司自主研发，拥有自主知识产权。公司拥有自组研发团队，拥有世界先进的重组生物技术；自主研发的重组生物用酶为世界上生物用酶的首创！基因工程羧肽酶B和基因工程胰蛋白酶是由基因工程方法生产，纯度高，不含杂酶，无羧肽酶A、糜蛋白酶等其他蛋白酶活性；无动物源性，出口产品无需进行终产品的动物源性检验。制品中不含蛋白酶抑制剂，产品安全。 公司秉承“科技致胜”的理念，坚持“提供高纯度重组蛋白酶”，并愿与广大同行交流，有需求的客户提供优质的服务！ 产品经多家医药公司检验和试用，认为符合重组人胰岛素生产的要求（5L和50L重组人胰岛素的生产），在纯度上高于sigma公司的产品（HPLC法）；在比活上与sigma公司的产品类似。
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详细地址：
上海市徐汇区银都路466号1号楼101室
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492549087
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