Recombinant human Trypsin--性能参数，报价/价格，图片

基本信息

产品名称：

Recombinant human Trypsin

英文名称：

Recombinant human Trypsin

国产/进口：

国产

产地/品牌：

YaxinBio

型号：

RHT03

参考报价：

销售商：

上海雅心生物技术有限公司

总点击数：

2033

更新日期：

2023-08-24

产品类别：

生化试剂

性能参数

Recombinant Human Trypsin

Catalog Number:RHT03

CAS: 9002-07-7
EC: 3.4.21.4

Storage temperature: 2-8℃

Source: Recombinant human trypsin is a genetically engineered protein expressed in E.coli

Protein Sequence:The amino acid sequence of recombinant human trypsin is identical to human pancreas-derived trypsin

1.DESCRIPTION

Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. YaxinBio recombinant human trypsin is a genetically engineered protein expressed in E.coli and the amino acid sequence is identical to human pancreas-derived trypsin,with equivalent properties compared to native trypsin .The enzyme is inhibited by serine protease inhibitors, e.g. PMSF,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.

2.MAIN FEATURES

Source	Recombinant E. coli
Appearance	White or off white powder
Specific activity	≥2500 USP units/mg pro.
Protein Content	≥ 90%
Purity(HPLC)	≥ 95%

UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A₂₅₃ of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).

3.RECOMMEND USAGE

Prepare 1-10mg/ml recombinant human trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-11.0.

4.STABILITY OF STORAGE AND TRANSPORT

Stability of storage: Recombinant human trypsin lyophilized should be stored under 2-8℃ in sealed container.It is stable within 24 months.After dissolved with 1mM HCl , it should be stored under -20℃. It is no activity loss after 10 times repeated freezing and thawing.
Stability of transport: The product is stable by blue ice insulation transport.

5.PRODUCT ADVANTAGE

Animal origin free: Recombinant human trypsin is no exogenous virus contamination,and any animal origin material is not used in the production process.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

6.PRODUCT USAGE

Recombinant human trypsin is an endopeptidase that can be used for lysine and arginine C-terminal cleavage of peptide bonds to cleave macromolecules into small peptides. Recombinant human trypsin is widely used in a variety of biotechnological processes, such as: cell culture cell separation of various tissues; degradation of denatured proteins; enzymatic hydrolysis, sequencing; stem cell therapy; tumor cell therapy.

7.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant trypsin

Recombinant enterokinase

公司简介

上海雅心生物技术有限公司主要从事基因工程蛋白酶的研发和生产，目前的主要品种为基因工程羧肽酶B和基因工程胰蛋白酶；为本公司自主研发，拥有自主知识产权。公司拥有自组研发团队，拥有世界先进的重组生物技术；自主研发的重组生物用酶为世界上生物用酶的首创！基因工程羧肽酶B和基因工程胰蛋白酶是由基因工程方法生产，纯度高，不含杂酶，无羧肽酶A、糜蛋白酶等其他蛋白酶活性；无动物源性，出口产品无需进行终产品的动物源性检验。制品中不含蛋白酶抑制剂，产品安全。公司秉承“科技致胜”的理念，坚持“提供高纯度重组蛋白酶”，并愿与广大同行交流，有需求的客户提供优质的服务！产品经多家医药公司检验和试用，认为符合重组人胰岛素生产的要求（5L和50L重组人胰岛素的生产），在纯度上高于sigma公司的产品（HPLC法）；在比活上与sigma公司的产品类似。

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