Recombinant Protein A--性能参数，报价/价格，图片

基本信息

产品名称：

Recombinant Protein A

英文名称：

Recombinant Protein A

国产/进口：

国产

产地/品牌：

雅心

型号：

RSPA05

参考报价：

销售商：

上海雅心生物技术有限公司

总点击数：

1569

更新日期：

2023-05-24

产品类别：

生化试剂

性能参数

Recombinant Protein A

Catalog Number:RSPA05

CAS:91932-65-9

Storage temperature: 2-8℃

Source:Protein A mutant of Staphylococcus aureus , expressed in E. coli

1.DESCRIPTION

Protein A is a membrane protein of Staphylococcus aureus and can specifically bind to antibodies. Protein A affinity chromatography columns have become widely used as affinity columns for purified antibodies from ascites, serum and cell culture isolated or purified extracts of antibodies from different subtypes of mammals or genetically engineered recombinant proteins containing antibody Fc fragments. alkali protein A is our company independent research and development production, with independent intellectual property rights. Recombinant protein A is expressed by E. coli and purified by chromatography to give a purity of 95% or more. The purification process does not use antibody column affinity chromatography to avoid the possibility of incorporation of unrelated IgG in the product.

2.MAIN FEATURES

Appearance	White or off white powder
Solubility	Clear liquid
Bioburden	≤ 1 cfu/ mg
SDS-PAGE	Single major band
SDS-PAGE (MW)	25000±1000 Daltons
UV Spectrum	E275/250 ≥ 1.2
UV Spectrum	E0.1% at 275nm = 0.17-0.23
Purity (HPLC)	≥ 95%

3.STABILITY OF STORAGE AND TRANSPORT

（1）Stability of storage: Recombinant protein A lyophilized should be stored under 2-8℃ in sealed container.

It is stable within 24 months.When gluing even with PBS (pH7.4) dissolved,it is now with the current use.After dissolved with pure water, it should be stored under -20℃.

（2）Stability of transport: The product is stable by blue ice insulation transport.

4.PRODUCT ADVANTAGE

Non-antibody column purification, the product without any other antibodies such as IgG and other pollution.
SPA-25 is our own research and development products, pH tolerance range, can withstand 0.5 M NaOH and 0.5M HCl treatment, no degradation, antibody binding capacity unchanged.
The product has a specially designed link for the purification of the column, can be directly used for the preparation of protein A column.
Stable quality: Mass production can ensure stable and continuous batch production.It is no difference between the batch and the product quality is stable.
High purity: Higher specific activity.Host protein residues is less than the limits of biological products.
Lyophilized powder: The product is lyophilized powder and is easy to store and transport.
Compliance with regulatory requirements: Production equipment and production environment comply with relevant regulatory requirements, and the production process is in full compliance with NSF ISO 9001: 2015 quality system and GMP guidelines.
Complete quality documents: we can provide relevant regular support files in according to customers’ requirement.

5.APPLICATION:

The C-terminus of the recombinant protein A contains cysteine which can be coupled to the solid separation medium for purification of the monoclonal antibody or polyclonal antibody. Recombinant protein A can be conjugated to a variety of reporter molecules (fluorescent dyes, enzyme labels, biotin, colloidal gold, radioactive labels, etc.) without affecting its binding to the antibody. These conjugated protein A derivatives can be used for antibody detection of Western-blot, ELISA, immunohistochemistry.

6.RELATED PRODUCTS

Recombinant carboxypeptidase B

Sequencing grade trypsin

Recombinant human trypsin

Recombinant enterokinase

公司简介

上海雅心生物技术有限公司主要从事基因工程蛋白酶的研发和生产，目前的主要品种为基因工程羧肽酶B和基因工程胰蛋白酶；为本公司自主研发，拥有自主知识产权。公司拥有自组研发团队，拥有世界先进的重组生物技术；自主研发的重组生物用酶为世界上生物用酶的首创！基因工程羧肽酶B和基因工程胰蛋白酶是由基因工程方法生产，纯度高，不含杂酶，无羧肽酶A、糜蛋白酶等其他蛋白酶活性；无动物源性，出口产品无需进行终产品的动物源性检验。制品中不含蛋白酶抑制剂，产品安全。公司秉承“科技致胜”的理念，坚持“提供高纯度重组蛋白酶”，并愿与广大同行交流，有需求的客户提供优质的服务！产品经多家医药公司检验和试用，认为符合重组人胰岛素生产的要求（5L和50L重组人胰岛素的生产），在纯度上高于sigma公司的产品（HPLC法）；在比活上与sigma公司的产品类似。

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