Recombinant Trypsin(Porcine)
Recombinant Trypsin
Catalog Number:RPT0201
CAS: 9002-07-7
EC: 3.4.21.4
Source: Porcine trypsin,expressed in E.coli.
DESCRIPTION
Trypsin is a member of the serine protease family. Trypsin cleaves peptides on the C-terminal end of lysine and arginine amino acid residues. The optimum pH of trypsin is pH 7.0-9.0. The enzyme is inhibited by serine protease inhibitors, e.g. PMSF ,aprotinin,soybean trypsin inhibitor and by metal chelating agents, e.g.EDTA.It is typically isolated from the pancreas of animals.Animal-derived materials are now subject to more strigent regulations, as authorities strive to ensure safer biologics and pharmaceuticals,such as in insulin and vaccine production.
Recombinant porcine trypsin is a genetically engineered protein expressed in E.coli and purified by high pressure liquid chromatography. Recombinant trypsin is expressed as active protease , with equivalent properties compared to native trypsin .It is manufactured under NSF ISO 9001-2008 and in accordance to good manufacturing practice (GMP) guidelines.No animal derived products are used in the fermentation,purification,or final formulation.
ADVANTAGES
Animal origin free:No animal-derived material is used during production.
Stablility:A sterile recombinant trypsin lyophilized powder eliminates the contamination risks and decreases the chance of activity loss in the process of transport and storage.
High purity:
1) Recombinant porcine trypsin provides increased specific activity no less than 3800 USP units/mg pro.
2) Reduce production costs while improving manufacturing progresses.
MAIN FEATURES
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Source |
E. Coli |
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Purified by |
HPLC |
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Format |
White lyophilized powder |
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Specific activity |
≥3800 USP units/mg pro |
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Purity（RP-HPLC) |
NLT 70% β-trysin, NMT 20% α-trypsin |
UNIT DEFINITION：One USP unit of trypsin activity will produce a Delta A253 of 0.003 per minute in a reaction volume of 3.2ml at pH7.6 and 25℃, with BAEE as a substrate (1cm light path).
RECOMMEND USAGE
Prepare 1-10mg/ml recombinant trypsin with 1mM HCl.The ratio to aimed protein is 1:50 to 1:1000 (w/w).The optimum pH is pH7.0-9.0.
STORAGE INSTRUCTIONS
Recombinant trypsin lyophilized should be stored under 2℃-8℃ in sealed container. It is stable within 24 months.After dissolved, it should be stored under -20℃. It is stable within 24 months and above 90% activity remained after 10 times repeated freezing and thawing.
E-mail：sales@yaxinbio.com
上海雅心生物技术有限公司主要从事基因工程蛋白酶的研发和生产，目前的主要品种为基因工程羧肽酶B和基因工程胰蛋白酶；为本公司自主研发，拥有自主知识产权。公司拥有自组研发团队，拥有世界先进的重组生物技术；自主研发的重组生物用酶为世界上生物用酶的首创！基因工程羧肽酶B和基因工程胰蛋白酶是由基因工程方法生产，纯度高，不含杂酶，无羧肽酶A、糜蛋白酶等其他蛋白酶活性；无动物源性，出口产品无需进行终产品的动物源性检验。制品中不含蛋白酶抑制剂，产品安全。 公司秉承“科技致胜”的理念，坚持“提供高纯度重组蛋白酶”，并愿与广大同行交流，有需求的客户提供优质的服务！ 产品经多家医药公司检验和试用，认为符合重组人胰岛素生产的要求（5L和50L重组人胰岛素的生产），在纯度上高于sigma公司的产品（HPLC法）；在比活上与sigma公司的产品类似。
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