G-418Sulfate(G418)
详细介绍 |
详细信息 规格 Gibco 11811-023 数量 常备现货 说明 瓶 产品详细参数名称:G-418 Sulfate (G418) 产品货号:Gibco 11811-023 规格:5G 10G 产地: Gibco 品牌商标: Gibco 供应商:鲁汶生物 数量:常备现货 英文名:G-418 Sulfate 保存条件:Storage at C 详细信息我公司提供的G418来自Gibco公司,货号11811-023,技术参数如下: Shipping Conditions GENETICIN is shipped at ambient temperature. Storage Conditions GENETICIN should be stored at room temperature, 15 to 30°C. GENETICIN is soluble in water and should be stored at 2 to 8°C as a solution. Background GENETICIN is an aminoglycoside related to Gentamicin and is used as a selective agent in molecular genetics experiments. It is toxic to both prokaryotic and eukaryotic cells. The resistance genes are dominant and are located on both Transposons Tn601 (903) and Tn5. The gene on Tn5 is referred to as a aminoglycoside phosphotransferase 3' (I) or APH(3')I.The genes are bacterial in derivation but they can be expressed in eukaryotic cells. Efficient expression is achieved when the neo DNA sequence is linked to eukaryotic DNA sequences that permit transcription and processing of the Neo coding sequence into mRNA. Introduction of either of these genes into cells can confer resistance to GENETICIN, enabling the cells to grow in media containing GENETICIN. Description GENETICIN is also known as Antibiotic G-418 Sulfate. GENETICIN is supplied as a white powder. The amount of GENETICIN required to be present in culture media to select for resistant cells varies with a number of factors including cell type. Good laboratory practice requires that the optimal concentration of GENETICIN required to maintain and select cells must be determined for each set of growth conditions. Invitrogen recommends that whenever experimental conditions are altered (including use of GENETICIN from a different lot), the optimal concentration of the product be re-evaluated. Quality Control Absorbance - Visible range: At a concentration of 100 mg/mL in water, GENETICIN gives a clear colorless solution. The absorbance of this solution at 400 nm is less than 0.075 O.D. Absorbance - Ultraviolet range: At a concentration of 1 mg/mL in water, the absorbance at 280 nm is less than 0.015 O.D. 产品详细描述产品促销价格: 1g ¥450 5g ¥2000 |
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