肝癌肿瘤分子标志物检测试剂盒

货号: BR-1027H    规格: 96 Assay      价格: ￥3926.00

Hepatocellular Carcinoma Molecular Marker (EGFR, PTK2, IGF1R) Detection Kit
 
Description: The kit provides a means of performing Hepatocellular Carcinoma detection method based on kinase activity assays. Three targets of molecular marker (EGFR, PTK2 and IGF1R) are involved in Hepatocellular Carcinoma. This kit includes Streptavidin Coated Plates and specific Substrate-Peptides (biotinylated peptide substrates) for detection of the activity of EGFR, PTK2 and IGF1R which indicate the prognosis of Hepatocellular Carcinoma.
 


Molecular Markers in Hepatocellular Carcinoma

 

Disease	Molecular Marker	Gene ID	Gene Disease Relation
Hepatocellular Carcinoma	EGFR	1956	marker/mechanism
Hepatocellular Carcinoma	PTK2	5747	marker/mechanism
Hepatocellular Carcinoma	IGF1R	3480	marker/mechanism

 

- Molecular markers for drug discovery and life science research (mechanism).


Peptide Core Sequence: 

Specific Substrate-Peptides for Hepatocellular Carcinoma Molecular Marker

 

Molecular Marker	Substrate-Peptide	Residue	Reaction_Type
EGFR	Peptides Mix	Tyr-743	Phosphorylation
PTK2	Peptides Mix	Tyr-40	Phosphorylation
IGF1R	Peptides Mix	Tyr-2	Phosphorylation

 

Label of Substrate-Peptides: Biotinylated.

Quality Control: The substrate peptide was selected using our Hepatocellular Carcinoma Diagnosis Kit. The quality of the biotinylated peptide was evaluated by reverse-phase HPLC and by mass spectrometry.

Storage: 

Store at 4 °C, Avoid Freeze.

Background:

EGFR (epidermal growth factor receptor) exists on the cell surface and is activated by binding of its specific ligands, including epidermal growth factor and transforming growth factor α (TGFα) (note, a full list of the ligands able to activate EGFR and other members of the ErbB family is given in the ErbB article). ErbB2 has no known direct activating ligand, and may be in an activated state constitutively or become active upon heterodimerization with other family members such as EGFR. Upon activation by its growth factor ligands, EGFR undergoes a transition from an inactive monomeric form to an active homodimer – although there is some evidence that preformed inactive dimers may also exist before ligand binding. PTK2 protein tyrosine kinase 2 (PTK2), also known as Focal Adhesion Kinase (FAK), PTK2 is a focal adhesion-associated protein kinase involved in cellular adhesion and spreading processes. FAK is phosphorylated in response to integrin engagement, growth factor stimulation, and the action of mitogenic neuropeptides. Integrin receptors are heterodimeric transmembrane glycoproteins that cluster upon ECM engagement leading to FAK phosphorylation and recruitment to focal adhesions. Between the amino and the carboxy regions lies the catalytic domain. Phosphorylation of the activation loop within this kinase domain is important for the kinase activity of FAK. The Insulin-like Growth Factor 1 (IGF-1) Receptor is a transmembrane receptor that is activated by IGF-1 and by the related growth factor IGF-2. It belongs to the large class of tyrosine kinase receptors. This receptor mediates the effects of IGF-1, which is a polypeptide protein hormone similar in molecular structure to insulin. IGF-1 plays an important role in growth and continues to have anabolic effects in adults – meaning that it can induce hypertrophy of skeletal muscle and other target tissues. Tyrosine kinase receptors, including the IGF-1 receptor, mediate their activity by causing the addition of a phosphate groups to particular tyrosines on certain proteins within a cell. This addition of phosphate induces what are called "cell signaling" cascades – and the usual result of activation of the IGF-1 receptor is survival and proliferation in mitosis-competent cells, and growth (hypertrophy) in tissues such as skeletal muscle and cardiac muscle.
 
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