EGFR磁性细胞分离试剂盒
基本信息
产品名称：
EGFR磁性细胞分离试剂盒
英文名称：
Anti-EGFR aptamer, Magnetic Cell Isolation Kit
国产/进口：
进口
产地/品牌：
英国 amsbio
型号：
4*1mL
参考报价：
销售商：
总点击数：
1406
更新日期：
2026-04-02
产品类别：
性能参数
产品名称:Anti-EGFR适体、磁性细胞分离试剂盒
产品目录号:EGFR-2369BCI(基于生物素化适配体的细胞分离试剂盒)
EGFR-2369FBCI(基于双标记适配体的细胞分离试剂盒)
内容:EGFR-2369BCI(生物素化适配体、链霉素包覆磁珠和缓冲液)，
EGFR 2369FBCI (FITC染料结合生物素适配体、链霉素包覆磁珠和缓冲液)
形态:干燥的适体，在50mm Tris pH8.0中的珠子, 150mm NaCl, 0.05% NaN3
适配子产生的蛋白来源:在哺乳动物细胞中产生的重组蛋白。
特异性:Anti-EGFR适配子结合到人的EGFR。没有测试与其他物种的交叉反应性。
MW:~ 18 kDa
测试应用程序:流式细胞仪和细胞隔离
运输和存储:在2°C到8°C条件下，1年内性能不会有影响
AptoPrepTM产品仅供研究使用。它们不用于医学、诊断或治疗用途。
描述
Amsbio提供两种类型的工具包。一种是基于生物素化适配子的细胞分离试剂盒。表皮生长因子受体- 2369 - bci)。基于适配体的磁性细胞分离试剂盒产品在分离过程中不会对细胞产生不利影响，因此可以用于分离纯的、活的和功能细胞，从而促进生物学研究。
EGFR细胞分离试剂盒是直接从各类样品中分离EGFR表达靶细胞的理想方法。也可以从含有试剂盒中释放缓冲液的珠细胞复合物中洗脱细胞，然后用于所有下游实验，包括流式细胞术、细胞培养和分子研究。
AptoPrepTM细胞分离原理
Amsbio细胞分离试剂盒是利用生物素化适配体和链霉亲和素磁珠间接分离细胞，并根据阳性选择原则设计的。靶向细胞是用生物素化适配体特异性地对所需要细胞的细胞表面靶向物进行标记。链霉亲和素磁珠允许有效结合到适配体标记的细胞。磁性标记的靶细胞然后用磁铁从未标记的细胞中分离出来。在细胞分离过程中，双标记适配体可立即进行流式细胞仪分析(目录号EGFR-2369FBCI)。最后一步，利用释放元件从珠结合细胞(阳性部分)中释放无珠和无适配体游离靶细胞。
阳性隔离;丢弃上清液，使用珠状细胞进行下游应用。
从珠子中释放目标细胞:用Kit中包含的释放缓冲液清洗珠子结合的细胞，从珠子中释放目标细胞。
下游应用程序
利用Amsbio EGFR细胞分离试剂盒可以有效地从样品中分离EGFR+细胞。分离后直接裂解细胞，分离出用于PCR、微阵列、蛋白质组学等不需要去除珠子的应用的蛋白质、DNA或mRNA。细胞因子表达、增殖/凋亡诱导等功能研究或流式细胞术分析，需在细胞阳性分离后从珠粒中释放细胞。试剂盒中包含的释放缓冲区将允许您收集无珠子和无适配体EGFR+细胞。EGFR+细胞经释放缓冲液洗脱后，用血球仪计数细胞计算洗脱产率，为51%。释放EGFR+细胞的纯度和活力分别为95.6%和79.7%。
英文
Product Information
Product name: Anti-EGFR aptamer, Magnetic Cell Isolation Kit
Catalog number: EGFR-2369BCI (biotinylated aptamer based cell isolation kit),
EGFR-2369FBCI (bi-labeled aptamer based cell isolation kit)
Content: EGFR-2369BCI (Biotinylated aptamer, streptavidin- coated magnetic bead and buffer),
EGFR-2369FBCI (FITC dye conjugated biotin aptamer, streptavidin-coated magnetic bead and buffer)
Protein source for generation of aptamer: Recombinantprotein produced in mammalian cells.
Specificity: Anti-EGFR aptamer binds to human EGFR.Cross reactivity with other species has not been tested.
MW: ~18 kDa
Tested applications: FACS and cell isolation
Shipping & Storage : At 2°C to 8°C. There is no decrease in performance of the kit after storage for 1 year at 2°C to 8°C.
Description
Amsbio provides two types of kits. One is biotinylated aptamer based cell isolation kit (Catalog No. EGFR-2369BCI). The other is bi-labeled (FITC dye conjugated biotin aptamer) aptamer based cell isolation kit (Catalog No. EGFR-2369FBCI). Aptamer based magnetic cell isolation kit products do not adversely affect cells during isolation process, thus can be used to isolate pure, viable and functional cells which advance your biology research. EGFR cell isolation kit is ideal for positive isolation of EGFR expressing target cells directly from all types of samples. Cell can also be eluted from bead-cell complexes with releasing buffer included in Kit, and then be used in all downstream experiments, including flow cytometry, cell culture and molecular studies.
Principle of the AptoPrepTM Cell Isolation
Amsbio cell isolation kit is designed to isolate cells via a indirect method and for positive selection principle using biotinylated aptamers and streptavidin magnetic beads. Target cells are specifically labeled with biotinylated aptamer against cell surface target of desired cells. Streptavidin magnetic beads allow for efficient binding to the aptamer labeled cell. Magnetically labeled target cells are then separated from unlabeled cells using magnet. FACS analysis can
immediately be performed with bi-labeled aptamer during cell isolation process (Catalog No. EGFR-2369FBCI). In final step, bead-free and aptamer-free target cells were released from bead[1]bound cells (positive fraction) using releasing component. Positive isolation: Discard the supernatants and use the bead[1]bound cells for downstream application. Release target cells from beads: Bead-bound cells are washed and target cells are released from the beads with releasing
buffer included in Kit.
Downstream application
EGFR+ cells can be efficiently isolated from a sample with Amsbio EGFR cell isolation kit. Lyse the cells directly after isolation, and isolate proteins, DNA, or mRNA to be used in PCR, microarrays, proteomics, and other applications where the removal of beads is not required. For functional studies such as cytokine expression, proliferation/apoptosis induction or for flow cytometry analysis, the cells need to be released from beads after positive isolation of cell. Releasing buffer included in Kit will allow you to collect the bead-free and aptamer-free EGFR+ cell.
After elution of EGFR+ cells with releasing buffer, elution yield was calculated by counting cells with hemocytometer and measured at 51% (data not shown). Purity and viability of released EGFR+ cells were measured at 95.6% and 79.7%, respectively
产品目录号:EGFR-2369BCI(基于生物素化适配体的细胞分离试剂盒)
EGFR-2369FBCI(基于双标记适配体的细胞分离试剂盒)
内容:EGFR-2369BCI(生物素化适配体、链霉素包覆磁珠和缓冲液)，
EGFR 2369FBCI (FITC染料结合生物素适配体、链霉素包覆磁珠和缓冲液)
形态:干燥的适体，在50mm Tris pH8.0中的珠子, 150mm NaCl, 0.05% NaN3
适配子产生的蛋白来源:在哺乳动物细胞中产生的重组蛋白。
特异性:Anti-EGFR适配子结合到人的EGFR。没有测试与其他物种的交叉反应性。
MW:~ 18 kDa
测试应用程序:流式细胞仪和细胞隔离
运输和存储:在2°C到8°C条件下，1年内性能不会有影响
AptoPrepTM产品仅供研究使用。它们不用于医学、诊断或治疗用途。
描述
Amsbio提供两种类型的工具包。一种是基于生物素化适配子的细胞分离试剂盒。表皮生长因子受体- 2369 - bci)。基于适配体的磁性细胞分离试剂盒产品在分离过程中不会对细胞产生不利影响，因此可以用于分离纯的、活的和功能细胞，从而促进生物学研究。
EGFR细胞分离试剂盒是直接从各类样品中分离EGFR表达靶细胞的理想方法。也可以从含有试剂盒中释放缓冲液的珠细胞复合物中洗脱细胞，然后用于所有下游实验，包括流式细胞术、细胞培养和分子研究。
AptoPrepTM细胞分离原理
Amsbio细胞分离试剂盒是利用生物素化适配体和链霉亲和素磁珠间接分离细胞，并根据阳性选择原则设计的。靶向细胞是用生物素化适配体特异性地对所需要细胞的细胞表面靶向物进行标记。链霉亲和素磁珠允许有效结合到适配体标记的细胞。磁性标记的靶细胞然后用磁铁从未标记的细胞中分离出来。在细胞分离过程中，双标记适配体可立即进行流式细胞仪分析(目录号EGFR-2369FBCI)。最后一步，利用释放元件从珠结合细胞(阳性部分)中释放无珠和无适配体游离靶细胞。
阳性隔离;丢弃上清液，使用珠状细胞进行下游应用。
从珠子中释放目标细胞:用Kit中包含的释放缓冲液清洗珠子结合的细胞，从珠子中释放目标细胞。
下游应用程序
利用Amsbio EGFR细胞分离试剂盒可以有效地从样品中分离EGFR+细胞。分离后直接裂解细胞，分离出用于PCR、微阵列、蛋白质组学等不需要去除珠子的应用的蛋白质、DNA或mRNA。细胞因子表达、增殖/凋亡诱导等功能研究或流式细胞术分析，需在细胞阳性分离后从珠粒中释放细胞。试剂盒中包含的释放缓冲区将允许您收集无珠子和无适配体EGFR+细胞。EGFR+细胞经释放缓冲液洗脱后，用血球仪计数细胞计算洗脱产率，为51%。释放EGFR+细胞的纯度和活力分别为95.6%和79.7%。
英文
Product Information
Product name: Anti-EGFR aptamer, Magnetic Cell Isolation Kit
Catalog number: EGFR-2369BCI (biotinylated aptamer based cell isolation kit),
EGFR-2369FBCI (bi-labeled aptamer based cell isolation kit)
Content: EGFR-2369BCI (Biotinylated aptamer, streptavidin- coated magnetic bead and buffer),
EGFR-2369FBCI (FITC dye conjugated biotin aptamer, streptavidin-coated magnetic bead and buffer)
Protein source for generation of aptamer: Recombinantprotein produced in mammalian cells.
Specificity: Anti-EGFR aptamer binds to human EGFR.Cross reactivity with other species has not been tested.
MW: ~18 kDa
Tested applications: FACS and cell isolation
Shipping & Storage : At 2°C to 8°C. There is no decrease in performance of the kit after storage for 1 year at 2°C to 8°C.
Description
Amsbio provides two types of kits. One is biotinylated aptamer based cell isolation kit (Catalog No. EGFR-2369BCI). The other is bi-labeled (FITC dye conjugated biotin aptamer) aptamer based cell isolation kit (Catalog No. EGFR-2369FBCI). Aptamer based magnetic cell isolation kit products do not adversely affect cells during isolation process, thus can be used to isolate pure, viable and functional cells which advance your biology research. EGFR cell isolation kit is ideal for positive isolation of EGFR expressing target cells directly from all types of samples. Cell can also be eluted from bead-cell complexes with releasing buffer included in Kit, and then be used in all downstream experiments, including flow cytometry, cell culture and molecular studies.
Principle of the AptoPrepTM Cell Isolation
Amsbio cell isolation kit is designed to isolate cells via a indirect method and for positive selection principle using biotinylated aptamers and streptavidin magnetic beads. Target cells are specifically labeled with biotinylated aptamer against cell surface target of desired cells. Streptavidin magnetic beads allow for efficient binding to the aptamer labeled cell. Magnetically labeled target cells are then separated from unlabeled cells using magnet. FACS analysis can
immediately be performed with bi-labeled aptamer during cell isolation process (Catalog No. EGFR-2369FBCI). In final step, bead-free and aptamer-free target cells were released from bead[1]bound cells (positive fraction) using releasing component. Positive isolation: Discard the supernatants and use the bead[1]bound cells for downstream application. Release target cells from beads: Bead-bound cells are washed and target cells are released from the beads with releasing
buffer included in Kit.
Downstream application
EGFR+ cells can be efficiently isolated from a sample with Amsbio EGFR cell isolation kit. Lyse the cells directly after isolation, and isolate proteins, DNA, or mRNA to be used in PCR, microarrays, proteomics, and other applications where the removal of beads is not required. For functional studies such as cytokine expression, proliferation/apoptosis induction or for flow cytometry analysis, the cells need to be released from beads after positive isolation of cell. Releasing buffer included in Kit will allow you to collect the bead-free and aptamer-free EGFR+ cell.
After elution of EGFR+ cells with releasing buffer, elution yield was calculated by counting cells with hemocytometer and measured at 51% (data not shown). Purity and viability of released EGFR+ cells were measured at 95.6% and 79.7%, respectively
公司简介
普瑞麦迪（北京）实验室技术有限公司坐落于中关村科技园丰台园区，是致力于生命科学实验室整体解决方案的国家高新技术企业。公司秉承“持续创新、以人为本、服务至上、合作共赢”的理念，以“引进全球创新技术、创新产品、助力中国生命科学飞速发展”为使命；聚集了一批行业内的专业精英，包括杰出的留学归国研发和管理人员及国内优秀人才等一起构成公司的精英团队。公司集自主研发和引进国际顶尖技术产品为一体，主营产品涵盖生命科学、医学、环境生物学及化学等领域的高端仪器及试剂。
售后服务
普瑞麦迪公司是专业的流式细胞仪代理和服务商，产品涵盖细胞成像系统、流式细胞仪、分子生物学产品、常规实验室设备及试剂等众多产品。公司有完善的售前、售后、实验室技术团队，有充足的仪器配件、DEMO样机及优秀的技术人员,“服务为本”是普瑞麦迪公司一贯的工作理念。
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