NutriFreez^® D10 Cryopreservation Medium is a ready-to-use, animal component-free, serum-free, and protein-free cell freezing solution optimized for multiple cell lines.  When culturing cells in a serum-free environment, it is essential to also maintain serum-free conditions during cryopreservation.  

NutriFreez^® D10 Cryopreservation Medium has been demonstrated to result in high rates of cell viability, proliferation, adherence (in relevant lines), and bioactivity/ expression following freezing and thawing.  Superior results were obtained both in comparison with serum-containing freezing media as well as competing serum-free products, making this an ideal product for both serum-containing and serum-free applications.

NutriFreez^® D10 Cryopreservation Medium has been extensively tested on multiple cell types, including:

• Human Mesenchymal Stem Cells (hMSC), from various sources (e.g. bone marrow (BM-MSC), adipose tissue (AT-MSC), umbilical cord tissue (UC-MSC) derived and dental pulp tissue (hMSC –DP)

• Human Embryonic Stem Cells (hESC), Induced Pluripotent Stem Cells (iPSC)

• Human Peripheral Blood Mononuclear Cells (PBMC’s)

• Human Endothelial Cells (EC)

• T cells, including Chimeric Antigen Receptors (CART) cells and Tumor-Infiltrating Lymphocytes (TIL’s)

• Neuron Cells

• Hybridomas

• CHO cells

• Vero Cells

• Multiple mammalian cell lines: MRC-5, HEK-293, HepG2, HeLaBSC-1, BGM3T3, MA-10BHK-21 as well as other extremely sensitive cell types

• Cryoprotective formulation designed to minimize dehydration effects

• Effective maintenance of cell viability, adhesion, and bioactivity

• Ready-to-use solution, simple protocol

• Suitable for use in cell banks and biopharmaceutical applications

• Animal component-free, serum-free, protein-free solution

• cGMP Manufactured

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