永生化人肾上皮细胞
| BioSafety Level | II |
| Organism | Homo sapiens |
| Source Organ | Renal |
| Growth Properties | Adherent |
| Morphology | Polygonal |
| Recommended Seeding Density | Thaw entire contents into an appropriate T25 flask as specified in the Propagation instructions. |
| Applications | For Research Use Only |
| Procedure Overview | ![]() |
| Propagation | Use of PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) is required for cell adhesion to the culture vessels. Grow cells in ECM-coated culture vessels with the following conditions. The base medium for this cell line is Prigrow X series medium available in ABM, Cat. No. TM0616. Atmosphere: air, 95%; Carbon dioxide (CO2), 5%. |
| Quality Control | Real Time PCR was used to quantify the transgene expression. |
| Disclaimer | 1. The CoA for this product (provided upon request) verifies the cell-type specific gene expression via RT-PCR only. All test parameters provided in the CoA are conducted using abm's standardized culture system and procedures. The stated values may vary under the end-user's culture conditions. Please verify that the product is suitable for your studies by referencing published papers or ordering RNA (0.5 μg, Cat.# C207, $450.00) or cell lysate (100 μg, Cat.# C206, $600.00) to perform preliminary experiments, or alternatively use our Gene Expression Assay Service (Cat# C138). All sales are final. 2. We strongly recommend live cell shipments for ease of cell transfer and this option can be requested at the time of ordering. Please note that the end-user will need to evaluate the feasibility of live cell shipment by taking into account the final destination's temperature variation and its geographical location. In addition, we thoroughly test our cell lines for freeze-thaw recovery. If frozen cells were received and not recovered in your lab under the exact, specified conditions (using recommended culture vessel, media, additional supplements, and atmospheric conditions), a live cell replacement is possible at a cost (plus shipping). 3. All of abm's cell biology products are for research use ONLY and NOT for therapeutic/diagnostic applications. abm is not liable for any repercussions arising from the use of its cell biology product(s) in therapeutic/diagnostic application(s). Please contact a technical service representative for more information. 4. abm makes no warranties or representations as to the accuracy of the information on this site. Citations from literature and provided for informational purposes only. abm does not warrant that such information has been shown to be accurate. |
镇江爱必梦生物科技有限公司成立于2016年,由李沛祥在江苏省镇江市创立,是一家位于国家大学科技园中心研发区的外商独资生物科技企业。
镇江爱必梦生物科技有限公司是加拿大AppliedBiologicalMaterials(abm)在中国的分公司。我们全面创新的产品系列涵盖了从CRISPR基因编辑工具、病毒载体及病毒,一直到全球最大最全的独特细胞系和最先进的PCR以及下一代测序技术及服务。同时,我们也有能力提供高度客制化的服务,使得研究人员能够从容面对各种困难和挑战。我们对于自身的市场定位是提供高质量、高性价比研究试剂的一站式服务型企业,满足科学界对于药物创新和生物医药市场的迫切需求。作为制造商和服务提供商,我们希望帮助科研人员简化常规科研程序,从而使科学家们得以把精力和时间投入到“大发现”中去,实现推动科学创新发展、提高科研效率的远大目标。
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