Cas9 Protein with NLS（Cas9 蛋白）
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Cas9 protein, in complex with guide RNA, offers benefit for CRISPR/Cas9 technology over plasmid or mRNA transfection. The biggest benefit is that you can make RNP complex that is ready to work in vivo. Cas9 protein is short lived (about 24 hours of half life in cells), thus minimize the off-target effects and mosaicism. You can also easily test the activity of guide RNA in vitro by a simple cleavage assay. In addition, Cas9 protein is pretty stable, and activity confirmation is much easier compared to mRNA. Lastly, it is active in more broad range of organisms such as nematodes, insects, plants, and non-typical model systems as it does not require transcription or translation.
Cas9 protein offered by PNA Bio is conjugated with NLS, and it is injection/transfection ready form.
Cy3-Cas9 can be an easy enrichment of the cells that are transfected with CRISPR/Cas9 complex.
精赛顿科技是由生命科学、医学研究领域的一线实验人员创办。在经历了十多年的实验后，我们明白“实验”是所有研究的基础，但也耗时费力。对于初入门者有何处着手的困惑，对于高阶人员又有着产出效率低的烦恼。为此精赛顿同学本着为初学者解决困惑，帮高阶者提高效率的初衷，致力于做您身边的实验助手，让研究来的更方便些。
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