子宫内膜癌类器官试剂盒--性能参数，报价/价格，图片

基本信息

产品名称：

子宫内膜癌类器官试剂盒

英文名称：

Endometrial Cancer Organoid Kit

国产/进口：

国产

产地/品牌：

杭州/伯桢

型号：

bioGenous

参考报价：

销售商：

伯桢生物科技（杭州）有限公司

总点击数：

272

更新日期：

2022-09-22

产品类别：

血清/培养基

性能参数

Product Description:
bioGenous^TM Endometrial Cancer Organoid Kit is a chemically defined cell culture medium for the establishment and maintenance of human endometrial cancer organoids. Patient-derived cancer organoids recapitulate the genomic and pathological features of original tumors and therefore hold great promise for medical research and precision medicine.

技术参数

Product Information:

Component	Component Cat#	Volume	Storage & Stability
bioGenous^TM Endometrial Cancer Organoid Basal Medium	K2166-EC-A100/A500	100mL/500mL	4℃，12 months
bioGenous^TM Endometrial Cancer Organoid Supplement B (50x)	K2166-EC-B100/B500	2mL/10mL	-20℃, avoid repeated freeze-thaw cycles, 12 months
bioGenous^TM Endometrial Cancer Organoid Supplement C (250x)	K2166-EC-C100/C500	0.4mL/2mL	-20℃, avoid repeated freeze-thaw cycles, 12 months

Preparation of Endometrial Cancer Organoid Complete Medium
Use a sterile technique to prepare the endometrial cancer organoid complete medium. The following example is for preparing 10mL complete medium. If preparing other volumes, adjust accordingly.

Thaw Endometrial Cancer Organoid Supplement B(50x) and Endometrial Cancer Organoid Supplement C(250x) on ice. Mix thoroughly.
Once thawed, use immediately or aliquot and store at -20°C for not more than 10 months. After thawing the aliquots, use immediately. Do not re-freeze.
Add 200μL Endometrial Cancer Organoid Supplement B(50x) and 40μL Endometrial Cancer Organoid Supplement C(250x) to 9.76mL Endometrial Cancer Organoid Basal Medium. Mix thoroughly.
If not used immediately, store the complete medium at 2-8°C for not more than 2 weeks. The Endometrial Cancer Organoid Supplement B contains fungicide and antibiotics (50x).

Protocol for Establishment of Patient-Derived Endometrial Cancer Organoids
CAUTION Studies involving primary human tissue material must follow all relevant institutional and government regulations. Informed consent must be obtained from all subjects before the collection of the primary human tissue material.
Establishment of Organoids from Primary Tissue

Collect primary human endometrial cancer tissue pieces in ice-cold Primary Tissue Storage Solution (K601005) with conical tubes. Keep tissue samples at 4°C until the start of the isolation.
Assess whether the obtained tissue pieces consist purely of epithelium. If fat or muscle tissues are present, remove these non-epithelial components as much as possible using surgical scissors or scalpels and forceps under a dissection microscope. If no fat or muscle tissues are present, continue to the next step immediately.
Rinse the tissue with Cancer Organoid Basal Medium (B213152) or DPBS twice.
Mince the tissue into small fragments of 1-3 mm³ in a cell culture dish using surgical scissors or scalpels.
Digest the tissue fragments with 10mL of Tumor Tissue Digestion Solution (K601003) in a 15mL conical tube at 37°C, with variable incubation times ranging from 30 min to 1 h. Carefully monitor the digestion process, mixing the content of the tube every 5-10 min by shaking vigorously or pipetting the mixture up and down. The digestion process could be finished when most of tissue fragments are able to pass through the 1mL pipette tips.
Add FBS to the tissue digestion mixture to a final concentration of 2%, and filter using a 100 μm cell strainer.
Collect and centrifuge the filtered cells at 250g for 3 min at 4 °C. In case of a visible red pellet, aspirate the supernatant, and resuspend the pellet using 2mL of Red Blood Cell Lysis Solution (E238010) to lyse the erythrocytes at room temperature for 1 min and centrifuge at 250g for 3 min at 4°C.
Aspirate the supernatant and resuspend the pellet in Cancer Organoid Basal Medium, centrifuge at 250g for 3 min at 4°C, repeat this step once more time.
Aspirate the supernatant and resuspend the pellet in Matrigel. The Matrigel should be kept on ice to prevent it from solidifying. Perform the process as quickly as possible. The amount of Matrigel used depends on the size of the pellet. Approximately 10,000 cells should be plated in 25 μL of Matrigel.
Do not overly dilute the Matrigel (Matrigel should be >70% (Matrigel vol/Total vol)), as this may inhibit the proper formation of the solid droplets.
Plate the Matrigel containing organoids at the bottom of 24-well cell culture plates in droplets of ~30 μL each around the center of the well..
Once the organoids are resuspended in Matrigel, proceed with plating as quickly as possible, as the Matrigel may solidify in the tube or pipette tip. Do not let the Matrigel touch the wall of wells.
Place the culture plate into a humidified incubator at 37 °C and 5% (vol/vol) CO₂ for 15-25 min to let the Matrigel solidify.
Prepare the required amount of endometrial cancer organoid complete medium.
Once the Matrigel droplets have solidified (15-25 min), open the plate and carefully add 500 μL of organoid complete medium to each well.
Do not add the medium directly on top of the Matrigel droplets, as this might disrupt the droplets.
Place the culture plate in a humidified incubator at 37 °C and 5% (vol/vol) CO₂.
Change the medium every 3-4 d by carefully aspirating the medium from the wells and replacing it with a fresh, pre-warmed organoid complete medium.
Closely monitor the organoid formation. Ideally, patient-derived endometrial cancer organoids should be passaged for the first time between 7 and 10 d after the initial plating.

Splitting and Passaging of Organoids

Pipette up and down to disrupt the Matrigel and transfer the organoid suspension to a 1.5 mL conical tube.
Pipette the organoid suspension up and down to mix thoroughly by pipetting against the bottom of the tube to create pressure, which will aid the removal of Matrigel.
Centrifuge organoids at 250g for 3 min at room temperature.
Aspirate the supernatant and split organoids using either Organoid Dissociation Solution (E238001) or by mechanical disruption. For Organoid Dissociation Solution-based cell dissociation, resuspend the pellet in 0.2 mL of Organoid Dissociation Solution, pipette up and down and incubate at 37 °C until organoids fall apart. Pipette up and down with a filter tip for ≥8 times every 2 min to aid in the disruption of the organoids. Closely monitor the digestion to keep the incubation time in the Organoid Dissociation Solution to a minimum. In case of mechanical disruption, resuspend the pellet in 1.5 mL of Cancer Organoid Basal Medium. Carefully pipette the organoid suspension up and down 30 times by pipetting against the bottom of the tube to create pressure, which will aid organoid disruption.
Do not dissociate in Organoid Dissociation Solution for >7 min, as this may result in poor organoid outgrowth or even loss of the culture. As a rule of thumb, digestion is complete if a mixture of small lumps of cells (consisting of 10–50 cells) can be observed.
After shearing is complete, wash once by topping up with 1 mL of Cancer Organoid Basal Medium, and centrifuge at 250g for 3 min at room temperature.
Aspirate the supernatant and resuspend the organoid pellet in 70% (vol/vol) Matrigel, and plate organoids in droplets at the bottom of a culture plate as described in Steps 10. After plating is complete, transfer the plate into a humidified incubator at 37 °C and 5% (vol/vol) CO₂ for 15–25 min.
Pre-warm endometrial cancer organoid complete medium at 37 °C.
After the Matrigel droplets have solidified (15–25 min), carefully pipette pre-warmed medium into the wells.

Place culture plates in a humidified incubator at 37 °C and 5% (vol/vol) CO₂ until the organoids are needed for further experiments.

公司简介

bioGenous（伯桢生物）致力于为生物医学科学研究、药物研发和临床精准医疗提供标准化、模块化类器官产品和技术支持。目前已服务200余家新药研发企业、三甲医院、科研院所，为用户提供类器官自动化设备、多组织全流程试剂盒、类器官培养和样本库构建技术方案、类器官药物研发和精准医疗子系统等。以国际领先、自主知识产权的类器官技术，打造行业上游独角兽，引领生物医学多领域模型革命。

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