Zeocin 2520.00元/1g Invitrogen产品 货号:R250-01
Description
Zeocin is an antibiotic in the bleomycin family. Its mode of action is to intercalate into the DNA and degrade it. The Zeocin resistance gene encodes a 13,665 dalton protein. This protein confers resistance to Zeocin by binding to the antibiotic and preventing it from binding DNA. Zeocin is effective on most aerobic cells and can be used for selection in mammalian cell lines, yeast, and bacteria. For Zeocin to be active, the salt concentration in the selection media must be less than 90 mM NaCl. The amount of Zeocin required for selection will vary with each cell type.
Concentration of Zeocin for Selection:
E. coli:25 µg/ml in low salt LB medium
Mammalian Cells:500-1000 µg/ml (varies with cell line)
Contents & Storage
Zeocin is provided in a solution with a concentration of 100 mg/ml. Zeocin should be stored at -20C in the dark. When stored correctly, the drug is guaranteed for 6 months from the date of shipment.