台湾旭基病毒核酸纯化试剂盒VR100

英文名	中文名	品牌	货号	规格	价格
Virus DNA/RNA Extraction Kit II	病毒核酸纯化试剂盒	Geneaid Biotech	VR100	100T	2250

Virus DNA/RNA Extraction Kit II VR050/VR100/VR300
The Viral Nucleic Acid Extraction Kit II was designed specifically for efficient purification of viral DNA and viral RNA from cell-free samples such as serum, plasma, body fluids and the supernatant of viral infected cell cultures. The efficient glass fiber spin column system is optimized for nucleic acid purification from a wide variety of both DNA and RNA viruses such as HBV, CMV, HCV, HIV, and HTLV. 10¹-10⁹ copies of viral DNA/RNA can be purified from up to 200 µl samples within 20 minutes. The purified viral DNA/RNA can be used directly in qPCR and qRT-PCR assays.
 
Advantages (Cat. # VR050, VR100, VR300)
· High Sensitivity: virus RNA/DNA can be successfully extrac　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　　ted and detected from as low as 10E1 copy number!
· Purify virus DNA or virus RNA in 20 minutes!
· Sample Volume: up to 200 µl samples of plasma, serum, body fluids, supernatant of viral cell cultures
· Spin Columns: glass fiber membrane optimized for virus DNA and virus RNA purification
· Individually packaged virus spin columns and collection tubes, certified RNase and DNase-free
· Elution Volume: 50 µl
· Storage: dry at room temperature (15-25ºC)
Applications
RT-PCR/PCR, qPCR, qRT-PCR, Real-time PCR, Real-time RT-PCR, Automated Fluorescent DNA Sequencing, Next Generation Sequencing (NGS) 
Components
· VB Lysis Buffer
· AD Buffer
· W1 Buffer
· Wash Buffer
· RNase-free Water
· VB Columns
· 2 ml Collection Tubes
Quality Control
The quality of Viral Nucleic Acid Extraction Kit II is tested on a lot-to-lot basis according to Geneaid's ISO-certified quality management system by isolating viral DNA/RNA from a 200 µl serum sample.
 

病毒基因组DNA/RNA 提取试剂盒
仅供科研使用
产品编号
VR050, VR100, VR300
完整说明书(英文)下载
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1. 处理材料
取200 μl样本（例如血浆,血清,淋巴液或经病毒感染的细胞培养液）转移到1.5 ml微量离心管中.
注意：样本不足200 μl可加缓冲液PBS补足.加入400 μl缓冲液VB Lysis,使用涡旋振荡器振荡混匀,室温放置10分钟.
 
2. 吸附
加入450 μl缓冲液AD（使用前请先检查是否已加入无水乙醇),充分摇动混匀. 将吸附柱VB放入2 ml收集管中. 将600 μl所得溶液加入吸附柱VB中,以14-16,000×g离心1分钟,倒掉收集管中的废液,将吸附柱放回收集管中. 将剩余的溶液加入吸附柱VB中,以14-16,000×g离心1分钟,丢弃收集管,将吸附柱放入新的收集管中.
 
3. 漂洗
向吸附柱VB中加入400 μl漂洗液W1,以14-16,000 ×g离心30秒,倒掉收集管中的废液,将吸附柱放入收集管中. 向吸附柱VB中加入600 μl漂洗液Wash（使用前请先检查是否已加入无水乙醇),以14-16,000×g离心30秒,倒掉收集管中的废液,将吸附柱VB放入收集管中. 以14-16,000 ×g离心3分钟将吸附柱中残余的漂洗液去除.
注意:漂洗液中乙醇的残留会影响后续实验.
 
4. 洗脱
将吸附柱VB放入一个RNase-free的离心管中,向吸附膜中间位置悬空滴加50 μl洗脱缓冲液RNase-free water,室温放置3分钟后,以14-16,000 ×g离心1分钟收集病毒DNA/RNA溶液。