MP土壤DNA提取试剂盒 116560200、6560-200

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用于从土壤中的所有生物和其它环境样品基因组DNA提取。只需500mg的样品即可得到足够的DNA。

实验原理：
用特殊的裂解介质E处理样品，之后使用自旋过滤器基于硅吸附DNA原理的GENECLEAN®步骤进行纯化，得到的DNA可用于PCR、限制性酶切、电泳及其他下游实验。
产品特点： 
1、配合FastPrep®仪器裂解样品处理更快、重复性更好、产量更高；
2、适用于从不同类型土壤的微生物和动植物组织及各种环境样品中分离基因组DNA；
3、对不同来源的样品，都可以在60min内裂解分离DNA，配合FastPrep®仪器只需30min；
4、无须使用有害的有机试剂，试剂盒不含有毒有害的试剂成分；

MP土壤DNA提取试剂盒，可在30分钟之内，快速、有效地从土壤以及其他环境样品中提取基因组DNA。

MP土壤DNA提取试剂盒用于从土壤中的所有生物包括G⁺细菌、酵母菌、真菌、藻类、线虫类甚至真细菌的孢子、芽孢中提取基因组DNA。而且它还可以提取其他环境样品的DNA，如沉积物、排泄物、废水、雪水等。只需500mg的样品即可得到足够的DNA。实验原理为用特殊的裂解介质E处理样品，之后使用自旋过滤器基于硅吸附DNA原理的GENECLEAN®步骤进行纯化，得到的DNA可用于PCR、限制性酶切、电泳及其他下游实验。

适用研究范围
土壤样品（粘土[clay], 沙质土[sandy], 淤泥[silty], 泥煤[peaty], 白垩质土[chalky]和 壤土[loamy soils]），粪便样品，环境水样，废水样品，淤泥样品等环境样品
1. 能有效的处理土壤样品中一些很难处理的组分如： 真细菌孢子（eubacterial spores）、内芽孢（endospores）、格兰仕阳性菌（gram positive bacteria）、酵母（yeast）、线虫（nematodes）、海藻（algea）、真菌（fungi）等。
2. 样品处理过程中使用的 MT Buffer和Sodium Phosphate Buffer可在整个提取过程中有效的保护核酸，并最大限度的降低RNA污染。
3. 基于硅珠的纯化方法，可有效地去除腐植酸、多元酚等PCR抑制物
4. 纯化所得的DNA可直接用于PCR、酶切等后续实验之中。
5. 对腐植酸含量特别高的样品，附加额外的处理方法。

现货供应“MP公司土壤DNA提取试剂盒，50 preps/Kit 货号：116560200 或 6560-200”

产品英文资料：
1. Introduction to the FastDNA® SPIN Kit for Soil and the FastPrep® Instruments
The FastDNA® SPIN Kit for Soil quickly and efficiently isolates PCR-ready genomic DNAdirectly from soil samples in less than 30 minutes. Designed for use with the FastPrep®Instruments from MP Biomedicals, plant and animal tissues, bacteria, algae, fungi spores and other members of a soil population are easily lysed within 40 seconds. These benchtop devices use a unique, optimized motion to homogenize samples by multidirectional, simultaneous impaction with lysing matrix particles. FastPrep® Instruments provide an extremely quick, efficient and highly reproducible homogenization that surpasses traditional extraction methods using enzymatic digestion, sonication, blending, douncing and vortexing. Samples are placed into 2.0 ml tubes containing Lysing Matrix E, a mixture of ceramic and silica particles designed to efficiently lyse all soil organisms including historically difficult sources such as eubacterial spores and endospores, gram positive bacteria, yeast, algae, nematodes and fungi. Homogenization in the FastPrep® Instrument with Lysing Matrix E takes place in the presence of MT Buffer and Sodium Phosphate Buffer, reagents carefully developed to protect and solubilize nucleic acids and proteins upon cell lysis. These reagents work together to allow extraction of genomic DNA with minimal RNA contamination.
Following lysis, samples are centrifuged to pellet soil, cell debris and lysing matrix. DNA is purified from the supernatant with a silica-based GENECLEAN® procedure using SPIN filters. Eluted DNA is ready for PCR, restriction digest, electrophoresis and any other desired application.
2. Kit Components and User Supplied Materials
2.1 FastDNA® SPIN Kit for Soil Components
Lysing Matrix E 50x 2.0 ml tubes
Sodium Phosphate Buffer 60 ml
MT Buffer 8 ml
PPS Solution 25 ml
Binding Matrix 66 ml
SPIN Modules 50 each
Catch Tubes 50 each
Concentrated SEWS-M 12 ml
DES 20ml
BBS Gel Loading Dye 200 μl
User manual 1 each
MSDS 1 each
Certificate of Analysis 1 each
FastDNA® Spin Kit for Soil
2.2 User Supplied Materials
FastPrep® Instrument (see Section 9)
Microcentrifuge that can freely spin 2.0 ml tubes
Microcentrifuge tubes (2.0 ml and 1.5 ml)
Clean 15 ml tubes for DNA binding
Rotator or low-speed vortex
3. Important Considerations Before Use
3.1 Preparation of SEWS-M Wash Solution
The FastDNA® SPIN Kit for Soil contains a bottle with 12 ml of Concentrated SEWS-M Wash Solution. Before using this solution, add 100 ml of 100% ethanol and mark on the bottle label the date ethanol was added. Ensure that the bottle is securely closed to prevent evaporation, mix and store at room temperature.
3.2 Sample Lysis with the FastPrep® Instrument
The fill volume in the lysing matrix tube after the addition of the Sodium Phosphate and MT Buffers to the sample should allow sufficient air space in the sample tube for efficient FastPrep® Instrument processing. MP Biomedicals recommends using 500 mg of starting material as long as there is between 250 – 500 μl of empty space in the tube. Sample loss or tube failure may result from overfilling the matrix tube. The matrix tube caps must be secure, but not over-tightened, to prevent sample leakage. If the sample is too large for processing in a single tube, divide the sample and process using multiple tubes.
MP Biomedicals’ Lysing Matrix particles and tubes have been rigorously tested and validated in the FastPrep® Instrument. The use of other products with the FastPrep® Instrument is not recommended and may result in sample loss or instrument failure.
A single 40 second run at a speed setting of 6.0 in the FastPrep® Instrument is sufficient to lyse almost all samples. If the user experimentally determines that additional processing time is required, the sample should be incubated on ice in the Lysing Matrix E tube for at least 2 minutes between successive FastPrep® Instrument homogenizations to prevent overheating the sample and tube.
4. Safety Precautions
Binding Matrix contains components that, when in contact with human tissue, may cause irritation. Wear personal protective equipment to prevent contact with the skin or mucus membranes (gloves, lab coat, and eye protection). Consult the enclosed Material Safety Data Sheet for additional details.
5. Protocol
1. Add up to 500 mg of soil sample to a Lysing Matrix E tube.
NOTE: See section 3.2 for important guidelines.
2. Add 978 μl Sodium Phosphate Buffer to sample in Lysing Matrix E tube.
3. Add 122 μl MT Buffer.
4. Homogenize in the FastPrep® Instrument for 40 seconds at a speed setting of 6.0.
5. Centrifuge at 14,000 x g for 5-10 minutes to pellet debris.
NOTE: Extending centrifugation to 15 minutes can enhance elimination of excessive debris from large samples, or from cells with complex cell walls.
6. Transfer supernatant to a clean 2.0 ml microcentrifuge tube. Add 250 μl PPS(Protein Precipitation Solution) and mix by shaking the tube by hand 10 times.
7. Centrifuge at 14,000 x g for 5 minutes to pellet precipitate. Transfer supernatant to a clean 15 ml tube. NOTE: While a 2.0 ml microcentrifuge tube may be used at this step, better mixing and DNA binding will occur in a larger tube.
8. Resuspend Binding Matrix suspension and add 1.0 ml to supernatant in 15 ml tube.
9. Place on rotator or invert by hand for 2 minutes to allow binding of DNA. Place tube in a rack for 3 minutes to allow settling of silica matrix.
10. Remove and discard 500 μl of supernatant being careful to avoid settled Binding
Matrix.
11. Resuspend Binding Matrix in the remaining amount of supernatant. Transfer approximately 600 μl of the mixture to a SPIN™ Filter and centrifuge at 14,000 x g for 1 minute. Empty the catch tube and add the remaining mixture to the SPIN™ Filter and centrifuge as before. Empty the catch tube again.
12. Add 500 μl prepared SEWS-M and gently resuspend the pellet using the force of the liquid from the pipet tip.
NOTE: Ensure that ethanol has been added to the Concentrated SEWS-M.
13. Centrifuge at 14,000 x g for 1 minute. Empty the catch tube and replace.
14. Without any addition of liquid, centrifuge a second time at 14,000 x g for 2 minutes to “dry” the matrix of residual wash solution. Discard the catch tube and replace with a new, clean catch tube.
15. Air dry the SPIN™ Filter for 5 minutes at room temperature.
16. Gently resuspend Binding Matrix (above the SPIN filter) in 50-100 μl of DES (DNase/Pyrogen-Free Water).
NOTE: To avoid over-dilution of the purified DNA, use the smallest amount of DES required to resuspend Binding Matrix pellet.
NOTE: Yields may be increased by incubation for 5 minutes at 55˚C in a heat block or water bath.
17. Centrifuge at 14,000 x g for 1 minute to bring eluted DNA into the clean catch tube. Discard the SPIN filter. DNA is now ready for PCR and other downstream applications. Store at -20°C for extended periods or 4°C until use.

美国MP 土壤DNA提取试剂盒 FastDNA™ SPIN Kit for Soil

现货供应“MP公司 土壤DNA提取试剂盒，50 preps/Kit 货号：116560200 或 6560-200”

MP公司产品订购联系方式：
北京研易来科技有限公司
电话：①③⑤②①⑥⑦③⑧④⑨
QQ号：413340189

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