等电压依赖性阴离子通道（内参）--性能参数，报价/价格，图片

基本信息

产品名称：

等电压依赖性阴离子通道（内参）

英文名称：

VDAC1(Mitochondrial Loading Control)

国产/进口：

国产

产地/品牌：

雅吉生物

型号：

52251R

参考报价：

销售商：

上海雅吉生物科技有限公司

总点击数：

825

更新日期：

2025-09-25

产品类别：

生化试剂

性能参数

英文名称VDAC1(Mitochondrial Loading Control)

中文名称等电压依赖性阴离子通道（内参）重组兔单克隆抗体

别名voltage-dependent anion channel; hVDAC1; MGC111064; Outer mitochondrial membrane protein porin 1; Plasmalemmal porin; Porin 31 HL; Porin 31HL; Porin 31HM; VDAC 1; VDAC; VDAC1; Voltage dependent anion channel 1; Voltage dependent anion selective channel protein 1; VTA1_HUMAN.

产品类型内参抗体

研究领域细胞生物免疫学细胞凋亡通道蛋白线粒体

抗体来源Rabbit

克隆类型Monoclonal

克隆号9A7

交叉反应Human, Mouse, Rat,

产品应用WB=1:300-100 IHC-P=1:400-800 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分子量32kDa

细胞定位细胞浆细胞膜线粒体

性状Lyophilized or Liquid

浓度1mg/ml

免疫原Recombinant human VDAC1:1-100/283

亚型IgG

纯化方法affinity purified by Protein A

储存液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

产品介绍Voltage dependent anion selective channel protein 1 (VDAC/Porin) belongs to the eukaryotic mitochondrial porin family and forms a channel through the mitochondrial outer membrane and also the plasma membrane. The channel allows diffusion of small hydrophilic molecules; it adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation selective. VDAC/Porin expression is observed in the heart,liver and skeletal muscle.

Function:

Involved in the endosomal multivesicular bodies (MVB) pathway. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. Thought to be a cofactor of VPS4A/B, which catalyzes disassembles membrane-associated ESCRT-III assemblies. Involved in the sorting and down-regulation of EGFR (By similarity). Involved in HIV-1 budding.

Subunit:

Interacts with VPS4B. Interacts with CHMP1B. Interacts with CHMP2A; the interaction probably involves the open conformation of (polymerized) CHMP2A. May interact with CHMP3. Interacts with CHMP5; the interaction involves soluble CHMP5. Interacts with IST1.

Subcellular Location:

Cytoplasm. Endosome membrane; Peripheral membrane protein (Probable).

Similarity:

Belongs to the VTA1 family.

SWISS:

P21796

Gene ID:

7416

Database links:

Entrez Gene: 416320 Chicken

Entrez Gene: 282119 Cow

Entrez Gene: 7416 Human

Entrez Gene: 22333 Mouse

Entrez Gene: 397010 Pig

Entrez Gene: 83529 Rat

Entrez Gene: 334582 Zebrafish

Omim: 604492 Human

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

电压依赖性阴离子通道VDAC是存在于线粒体外膜上的31kDa蛋白,能在膜上形成亲水性通道,调控阴离子、阳离子、ATP以及其他代谢物进出线粒体,在调节细胞代谢、维持胞内钙稳态,调节细胞凋亡和坏死等过程中发挥重要功能。

产品图片

Sample:
Lane 1: Raji (Human) Cell Lysate at 30 ug
Lane 2: HepG2 (Human) Cell Lysate at 30 ug
Lane 3: SW480 (Human) Cell Lysate at 30 ug
Lane 4: MCF-7 (Human) Cell Lysate at 30 ug
Lane 5: Jurkat (Human) Cell Lysate at 30 ug
Lane 6: 293T (Human) Cell Lysate at 30 ug
Lane 7: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 8: Heart (Mouse) Lysate at 40 ug
Lane 9: Heart (Rat) Lysate at 40 ug
Lane 10: Liver (Mouse) Lysate at 40 ug
Lane 11: Liver (Rat) Lysate at 40 ug
Lane 12: Kidney (Mouse) Lysate at 40 ug
Lane 13: Kidney (Rat) Lysate at 40 ug
Lane 14: Muscle (Mouse) Lysate at 40 ug
Primary: Anti-VDAC1 (bsm-52251R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human esophageal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat small intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (mouse kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (VDAC1) Monoclonal Antibody, Unconjugated (bsm-52251R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

公司简介

上海雅吉生物科技有限公司成立于2011年3月，是一家面向生命科学领域，科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学，通过公司各个部门所有员工的共同努力在行业内拥有较高知名度，深得新老客户的厚爱，本着“优质、服务、信誉”的精神，坚持以先进的技术、优质的产品、良好的信誉，为国内外广大用户提供优质生物产品和服务。公司在重视产品质量的同时，也建立了一套集技术支持、物流、售后服务等多个部门联动服务体系，努力把我们方便、快捷、周到的服务提供给每一个客户本研究所郑重承诺：质量保证、供货及时、服务周到，公司总投资超过2000万元，并先后引进了自动化设备，组建了专业的研发技术团队。凭借着洁净化的生产车间、标准化的质量管理系统，为生产高质量的产品提供了有效保证。
雅吉生物自主研发的ELISA试剂盒，在国内众多重点实验室广泛使用，深受广大科研人员的好评，先后在权威杂志文章中被引用。
上海雅吉生物科技有限公司以极强的职业精神，积极、负责、坦诚的服务令我们的客户满意！
客户服务承诺
以“服务、客户、技术”为根本。客户是我们的核心，坚持以高质量的产品服务客户，秉持诚信原则，重视客户的意见与感受；我们愿意为大家提供良好的售后服务。希望老师们对我们的服务及工作提出意见，您的满意是我们工作的动力，祝广大师生客户生活工作愉快！
更多产品详情可查询官网：www.yajimall.com

售后服务

试剂盒售后：本公司出售的试剂盒均保质保量，质量问题均可免费退换，另提供免费代测服务。细胞售后： 1. 细胞运输丢失、瓶身破损、培养液严重漏液等，重发； 3. 细胞收到当天以及第2,3天请拍照，未告知的视为产品合格。4-10天内出现问题，请提供细胞照片和细胞出现问题的照片以及细胞相关操作的详细步骤，并跟我公司人员及时沟通判定是否重发，具体可以参照我官网或者随货说明书相关售后条款。