微管蛋白β1 tubulin(内参)单克隆抗体--性能参数，报价/价格，图片

基本信息

产品名称：

微管蛋白β1 tubulin(内参)单克隆抗体

英文名称：

beta I Tubulin(Loading Control)

国产/进口：

国产

产地/品牌：

雅吉生物

型号：

33041M

参考报价：

销售商：

上海雅吉生物科技有限公司

总点击数：

3955

更新日期：

2026-03-26

产品类别：

生化试剂

性能参数

英文名称beta I Tubulin(Loading Control)

中文名称微管蛋白β1 tubulin(内参)单克隆抗体

别名2810484G07Rik; Beta tubulin 1, class VI; Class VI beta tubulin; dJ543J19.4; M(beta)1; TBB1_HUMAN; TUBB1; Tubulin beta 1 class VI; Tubulin beta-1 chain; Tubulin, beta 1; tubulin, beta1;

产品类型内参抗体

研究领域细胞生物免疫学信号转导细胞骨架

抗体来源Mouse

克隆类型Monoclonal

克隆号5F7

交叉反应Human, Mouse, Rat,

产品应用WB=1:5000-20000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分子量50kDa

细胞定位细胞浆

性状Liquid

浓度1mg/ml

免疫原KLH conjugated synthetic peptide derived from human beta I Tubulin:

亚型IgG2a

纯化方法affinity purified by Protein G

储存液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存条件Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

PubMedPubMed

产品介绍This gene encodes a member of the beta tubulin protein family. Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. This protein is specifically expressed in platelets and megakaryocytes and may be involved in proplatelet production and platelet release. A mutations in this gene is associated with autosomal dominant macrothrombocytopenia. Two pseudogenes of this gene are found on chromosome Y.[provided by RefSeq, Jul 2010]

Function:

Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).

Subunit:

Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Interacts with RANBP10.

Subcellular Location:

Cytoplasm, cytoskeleton

Tissue Specificity:

Hematopoietic cell-specific. Major isotype in leukocytes, where it represents 50% of all beta-tubulins.

Post-translational modifications:

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866).

Some glutamate residues at the C-terminus are monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella). Both polyglutamylation and monoglycylation can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of monoglycylation is still unclear (Probable).

Phosphorylated on Ser-172 by CDK1 during the cell cycle, from metaphase to telophase, but not in interphase. This phosphorylation inhibits tubulin incorporation into microtubules.

DISEASE:

Macrothrombocytopenia, autosomal dominant, TUBB1-related (MAD-TUBB1). The disease is caused by mutations affecting the gene represented in this entry. A congenital blood disorder characterized by increased platelet size and decreased number of circulating platelets.

Similarity:

Belongs to the tubulin family.

SWISS:

Q9H4B7

Gene ID:

81027

Database links:

Entrez Gene: 396427 Chicken

Entrez Gene: 541271 Cow

Entrez Gene: 485950 Dog

Entrez Gene: 101836899 Hamster

Entrez Gene: 81027 Human

Entrez Gene: 545486 Mouse

Entrez Gene: 679312 Rat

Omim: 612901 Human

SwissProt: P09203 Chicken

SwissProt: Q9H4B7 Human

SwissProt: A2AQ07 Mouse

Unigene: 303023 Human

Unigene: 45285 Mouse

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

产品图片

Sample:
K562 Cell (Human) Lysate at 40 ug
Jurkat Cell (Human) Lysate at 40 ug
Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: Cerebrum (Rat) Lysate at 40 ug
Lane 3: Cerebrum (Mouse) Lysate at 40 ug
Lane 4: Fetal brain (Mouse) Lysate at 40 ug
Lane 5: Heart (Mouse) Lysate at 40 ug
Lane 6: SH-SY5Y (Human) Cell Lysate at 30 ug
Lane 7: Spleen (Rat) Lysate at 40 ug
Lane 8: Spleen (Mouse) Lysate at 40 ug
Lane 9: Ovary (Rat) Lysate at 40 ug
Primary: Anti-beta I Tubulin (bsm-33041M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Sample:
293T(Human) Cell Lysate at 30 ug
Cerebellum (Mouse) Lysate at 40 ug
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti- beta I Tubulin (bsm-33041M) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kD
Observed band size: 50 kD

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M 5F7) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Tissue/cell: HeLa cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (bsm-33041M) 1:50, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody (bs-0296G-FITC) at 37°C for 90 minutes, DAPI (5ug/ml, blue, C-0033) was used to stain the cell nuclei.

公司简介

上海雅吉生物科技有限公司成立于2011年3月，是一家面向生命科学领域，科研机构、高校、院所及企业产品开发研究提供所需要的科研类试剂、耗材、仪器、技术服务等。包括分子生物学、免疫学、微生物学、细胞学，通过公司各个部门所有员工的共同努力在行业内拥有较高知名度，深得新老客户的厚爱，本着“优质、服务、信誉”的精神，坚持以先进的技术、优质的产品、良好的信誉，为国内外广大用户提供优质生物产品和服务。公司在重视产品质量的同时，也建立了一套集技术支持、物流、售后服务等多个部门联动服务体系，努力把我们方便、快捷、周到的服务提供给每一个客户本研究所郑重承诺：质量保证、供货及时、服务周到，公司总投资超过2000万元，并先后引进了自动化设备，组建了专业的研发技术团队。凭借着洁净化的生产车间、标准化的质量管理系统，为生产高质量的产品提供了有效保证。
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售后服务

试剂盒售后：本公司出售的试剂盒均保质保量，质量问题均可免费退换，另提供免费代测服务。细胞售后： 1. 细胞运输丢失、瓶身破损、培养液严重漏液等，重发； 3. 细胞收到当天以及第2,3天请拍照，未告知的视为产品合格。4-10天内出现问题，请提供细胞照片和细胞出现问题的照片以及细胞相关操作的详细步骤，并跟我公司人员及时沟通判定是否重发，具体可以参照我官网或者随货说明书相关售后条款。