The Agilent Seahorse XF Flex analyzer measures and reports the oxygen consumption rate (OCR), proton efflux rate (PER) or extracellular acidification rate (ECAR), as well as ATP production rates in a 24-well format. It offers optimized workflows and dedicated consumables to enable real-time metabolic analysis with 3D study models, such as organoids and tissue materials. It also provides wide signal dynamic range through the use of dedicated consumables.

OCR, PER, and ATP production rates are critical attributes of energy metabolism and vital indicators of mitochondrial health, toxicity, glycolysis, and overall cellular (dys) function. The ability to analyze these parameters in more complex three-dimensional structures will reveal deeper insights and enhance the translatability of your research.

The XF Flex analyzer is also equipped with advanced software and advanced data analytics available in the Agilent Seahorse Analytics software. These features greatly simplify the entire XF assay experience, from assay design to data QC and interpretation.


特性

• A flexible 24-well system that allows real-time metabolic analysis with improved sensitivity and consistency, using two-dimensional (2D) and three-dimensional (3D) study models
• Multiple parameters reported from each assay well, including OCR, PER or ECAR, and ATP production rates
• Tailored workflows and dedicated consumables for working with organoids and tissue materials
• Wide detection range and great for high respiration study models
• Four-port injection system with automated mixing feature for assessing immediate cellular, tissue organoid responses to substrates, inhibitors, and other compounds in real time
• Verified instrument performance to ensure intraplate and interplate data consistency
• Broad operation environment temperature of 4 to 30 °C, allowing the analyzer to maintain internal assay temperature of 16 to 42 °C
• Intuitive, user-guided software for easy assay protocol creation
• Automated data quality check in data analysis software to flag abnormal assay behavior
• Data files compatible with advanced data analysis features in the Seahorse Analytics which is a secure web-based data analysis tool

 
性能指标
工作原理

实时监测微孔板中的活细胞生物能量代谢
线粒体呼吸和糖酵解这两个主要的能量产生途径，分别涉及细胞耗氧量和质子释放率。  Seahorse XF 技术使用无标记传感器检测这些分析物中的细胞外变化，以测定细胞呼吸率、糖酵解和 ATP 产生。该技术可用于研究培养细胞、悬浮细胞以及组织、类器官和/或多细胞簇等三维材料的代谢变化。
形成微室，并计算每分钟细胞外流量速率
仪器将探针板降低至检测孔中。传感器位于孔底上方特定距离处，形成非常小的瞬态微室，这种设计对实现 Seahorse XF 技术独有的高灵敏度检测至关重要。 随着氧气和 pH 水平的变化，仪器可记录传感器中的相应变化。测量时间通常为 3 分钟，并自动执行速率计算。每次测量结束后，升高探针，使细胞外培养基恢复到基础条件。 
最多注入 4 种化合物，实时测试响应或研究生物学机理
探针板还配置有加药口（每孔 4 个），可在分析过程中将调节因子注入细胞孔中。当完成仪器方案配置后，系统会将化合物“A”注入分析孔中，缓慢混合，确保化合物在分析培养基中均匀分布。所有孔以此方式同步处理。系统将自动执行后续测量周期、方案规定的任何额外加药及速率计算。 
应用

探索细胞代谢的强大功能
安捷伦 Seahorse XF 平台可实时测量活细胞的两个主要代谢通路（线粒体呼吸和糖酵解），提供细胞生物能量代谢的功能动力学测量。了解生物能量参数如何提供有价值的信息，并作为疾病模型、关键细胞过程和疗法发现的指标。  
免疫代谢
包括激活、增殖和记忆细胞发育在内的免疫细胞过程都是由代谢重编程驱动的，代谢重编程可以被调节以增强性能和控制免疫细胞结局。通过功能性实时代谢测量，了解激活、增殖和记忆细胞发育等免疫细胞过程。
癌症代谢
新陈代谢是癌症恶性肿瘤细胞生长的关键驱动因素，为了总体上向糖酵解表型转换，癌细胞增殖通常需要进行上调或“代谢转换”，从而加快能量需求并生成结构单元，最终促进癌细胞生长。通过对活细胞进行实时功能性生物能量代谢分析，揭示癌症代谢特性，更深入地了解癌细胞生物学。