人肝癌细胞
基本信息
性能参数
名称：人肝癌细胞（活细胞顺丰包邮， 或可供冻存管）
资源详情页网址信息：https://www.cellbank.org.cn/search-detail.php?id=150
细胞株详情信息：Hep G2细胞是从一名患有癌症的15岁白人男性青年的肝细胞癌中分离出来的一种具有上皮样形态的细胞系。该细胞系由Wistar Institute保藏，是合适的转染宿主。该细胞分泌多种血浆蛋白：清蛋白、α2-巨球蛋白、血纤维蛋白溶酶原、铁传递蛋白，表达3-羟基-3-甲基戊二酸辅酶A还原酶和肝甘油三酸脂脂肪酶。表达标记物也包括胰岛素；胰岛素样生长因子II（IGF II）。常用于药物代谢和肝毒性研究。 在本库通过支原体检测阴性（可供检测报告）。 细菌检测结果：阴性；真菌检测结果：阴性 在本库通过STR检测无误（可供检测报告）。 STR鉴定结果： Amelogenin: X,Y D5S818:11,12 D13S317: 9,13 D7S820: 10 D16S539: 12 vWA: 17 TH01: 9 TPOX: 8,9 CSF1PO: 10,11 D19S433: 15.2 D21S11: 29,31 D18S51: 13,14
动物种别：人 白人
性别：男性
组织来源：肝细胞癌
形态：上皮细胞，贴壁生长
培养基和添加剂：人肝癌细胞 Hep G2完全培养液（目录号：MD119）组分： DMEM培养基(GIBCO货号：10566016)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 培养参考：细胞消化时间5-10min（0.25%胰酶）。传代比例1:2，传代周期3-4天 冻存液配方：完全培养液92%，DMSO 8% 细胞运输方式：活细胞可保温运输或干冰冻存管。 注意事项： （1）Hep G2细胞呈块状生长。细胞传代消化一定要完全至细胞团块脱落培养瓶底部再终止消化，消化不完全的话，细胞容易聚集。 （2）细胞对血清敏感，需要高质量血清或购买配套完全培养液（MD02）
供应限制：A。仅供研究之用。
REFERENCE：Knowles BB , et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960 Knowles BB , Aden DP . Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983 Schardt C , et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141 Aden DP , et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137 Busch SJ , et al. Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3- methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2. J. Biol. Chem. 265: 22474-22479, 1990. PubMed: 2176219 Darlington GJ , et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851 Cuthbert C , et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. PubMed: 9169468 Deleersnyder V , et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401 Benn J , et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004 Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Lewis W , et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. PubMed: 8622980 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Roesler WJ , et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Lieber A , et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed: 8971024 Dubuisson J , Rice CM . Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615 Yamaguchi Y , et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Kounas MZ , et al. Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein. J. Biol. Chem. 271: 6523-6529, 1996. PubMed: 8626456 Klemm DJ , et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed: 8626493 Wu X , et al. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins. J. Biol. Chem. 271: 10277-10281, 1996. PubMed: 8626595 Ostlund RE Jr, et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 Cancer Research 63:3775-3782,2003 Chinese Journal of Cancer 21(3):267-271, 2002 Chin J Dig. 22(6):331-334,2002 Chin J Cancer Biother 9(3):175-178,2002 Natl Med J China 82(8):564-567,2002 Chinese Journal of Cancer 22(3):248-252,2003 Chinese Journal of Cancer 22(12):1280-1283,2003 The Practical Journal of Cancer 18(5):490-491,2003 Immunological Journal 19(3):208-210,2003 J Fujian Med Univ 37(2):162-165,2003 Shanghai Immunol Journal 23(1):16-19,2003
资源详情页网址信息：https://www.cellbank.org.cn/search-detail.php?id=150
细胞株详情信息：Hep G2细胞是从一名患有癌症的15岁白人男性青年的肝细胞癌中分离出来的一种具有上皮样形态的细胞系。该细胞系由Wistar Institute保藏，是合适的转染宿主。该细胞分泌多种血浆蛋白：清蛋白、α2-巨球蛋白、血纤维蛋白溶酶原、铁传递蛋白，表达3-羟基-3-甲基戊二酸辅酶A还原酶和肝甘油三酸脂脂肪酶。表达标记物也包括胰岛素；胰岛素样生长因子II（IGF II）。常用于药物代谢和肝毒性研究。 在本库通过支原体检测阴性（可供检测报告）。 细菌检测结果：阴性；真菌检测结果：阴性 在本库通过STR检测无误（可供检测报告）。 STR鉴定结果： Amelogenin: X,Y D5S818:11,12 D13S317: 9,13 D7S820: 10 D16S539: 12 vWA: 17 TH01: 9 TPOX: 8,9 CSF1PO: 10,11 D19S433: 15.2 D21S11: 29,31 D18S51: 13,14
动物种别：人 白人
性别：男性
组织来源：肝细胞癌
形态：上皮细胞，贴壁生长
培养基和添加剂：人肝癌细胞 Hep G2完全培养液（目录号：MD119）组分： DMEM培养基(GIBCO货号：10566016)，90%；优质胎牛血清，10%。 气相：空气，95%；二氧化碳，5%。 温度：37摄氏度。 培养参考：细胞消化时间5-10min（0.25%胰酶）。传代比例1:2，传代周期3-4天 冻存液配方：完全培养液92%，DMSO 8% 细胞运输方式：活细胞可保温运输或干冰冻存管。 注意事项： （1）Hep G2细胞呈块状生长。细胞传代消化一定要完全至细胞团块脱落培养瓶底部再终止消化，消化不完全的话，细胞容易聚集。 （2）细胞对血清敏感，需要高质量血清或购买配套完全培养液（MD02）
供应限制：A。仅供研究之用。
REFERENCE：Knowles BB , et al. Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497-499, 1980. PubMed: 6248960 Knowles BB , Aden DP . Human hepatoma derived cell line, process for preparation thereof, and uses therefor. US Patent 4,393,133 dated Jul 12 1983 Schardt C , et al. Characterization of insulin-like growth factor II receptors in human small cell lung cancer cell lines. Exp. Cell Res. 204: 22-29, 1993. PubMed: 8380141 Aden DP , et al. Controlled synthesis of HBsAg in a differentiated human liver carcinoma- derived cell line. Nature 282: 615-616, 1979. PubMed: 233137 Busch SJ , et al. Differential regulation of hepatic triglyceride lipase and 3-hydroxy-3- methylglutaryl-CoA reductase gene expression in a human hepatoma cell line, HepG2. J. Biol. Chem. 265: 22474-22479, 1990. PubMed: 2176219 Darlington GJ , et al. Growth and hepatospecific gene expression of human hepatoma cells in a defined medium. In Vitro Cell. Dev. Biol. 23: 349-354, 1987. PubMed: 3034851 Cuthbert C , et al. Regulation of human apolipoprotein A-I gene expression by gramoxone. J. Biol. Chem. 272: 14954-14960, 1997. PubMed: 9169468 Deleersnyder V , et al. Formation of native hepatitis C virus glycoprotein complexes. J. Virol. 71: 697-704, 1997. PubMed: 8985401 Benn J , et al. Hepatitis B virus HBx protein induces transcription factor AP-1 by activation of extracellular signal-regulated and c-Jun N-terminal mitogen-activated protein kinases. J. Virol. 70: 4978-4985, 1996. PubMed: 8764004 Goodrum FD , et al. Adenovirus early region 4 34-kilodalton protein directs the nuclear localization of the early region 1B 55-kilodalton protein in primate cells. J. Virol. 70: 6323-6335, 1996. PubMed: 8709260 Kolanus W , et al. alphaLbeta2 integrin/LFA-1 binding to ICAM-1 induced by cytohesin-1 a cytoplasmic regulatory molecule. Cell 86: 233-242, 1996. PubMed: 8706128 Lewis W , et al. Fialuridine and its metabolites inhibit DNA polymerase gamma at sites of multiple adjacent analog incorporation, decrease mtDNA abundance, and cause mitochondrial structural defects in cultured hepatoblasts. Proc. Natl. Acad. Sci. USA 93: 3592-3597, 1996. PubMed: 8622980 Jang SI , et al. Activator protein 1 activity is involved in the regulation of the cell type-specific expression from the proximal promoter of the human profilaggrin gene. J. Biol. Chem. 271: 24105-24114, 1996. PubMed: 8798649 Roesler WJ , et al. The alpha-isoform of the CCAAT/enhancer-binding protein is required for mediating cAMP responsiveness of the phosphoenolpyruvate carboxykinase promoter in hepatoma cells. J. Biol. Chem. 271: 8068-8074, 1996. PubMed: 8626491 Lee JH , et al. The proximal promoter of the human transglutaminase 3 gene. J. Biol. Chem. 271: 4561-4568, 1996. PubMed: 8626812 Lieber A , et al. Recombinant adenoviruses with large deletions geneerated by cre-mediated excision exhibit different biological properties compared with first-generation vectors in vitro and in vivo. J. Virol. 70: 8944-8960, 1996. PubMed: 8971024 Dubuisson J , Rice CM . Hepatitis C virus glycoprotein folding: disulfide bond formation and association with calnexin. J. Virol. 70: 778-786, 1996. PubMed: 8551615 Yamaguchi Y , et al. Biochemical characterization and intracellular localization of the Menkes disease protein. Proc. Natl. Acad. Sci. USA 93: 14030-14035, 1996. PubMed: 8943055 Kounas MZ , et al. Cellular internalization and degradation of antithrombin III-thrombin, heparin cofactor II-thrombin, and alpha1-antitrypsin-trypsin complexes is mediated by the low density lipoprotein receptor-related protein. J. Biol. Chem. 271: 6523-6529, 1996. PubMed: 8626456 Klemm DJ , et al. Adenovirus E1A proteins regulate phosphoenolpyruvate carboxykinase gene transcription through multiple mechanisms. J. Biol. Chem. 271: 8082-8088, 1996. PubMed: 8626493 Wu X , et al. Demonstration of a physical interaction between microsomal triglyceride transfer protein and apolipoprotein B during the assembly of ApoB-containing lipoproteins. J. Biol. Chem. 271: 10277-10281, 1996. PubMed: 8626595 Ostlund RE Jr, et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 Cancer Research 63:3775-3782,2003 Chinese Journal of Cancer 21(3):267-271, 2002 Chin J Dig. 22(6):331-334,2002 Chin J Cancer Biother 9(3):175-178,2002 Natl Med J China 82(8):564-567,2002 Chinese Journal of Cancer 22(3):248-252,2003 Chinese Journal of Cancer 22(12):1280-1283,2003 The Practical Journal of Cancer 18(5):490-491,2003 Immunological Journal 19(3):208-210,2003 J Fujian Med Univ 37(2):162-165,2003 Shanghai Immunol Journal 23(1):16-19,2003
公司简介
中国科学院细胞库简介
为了顺应我国生命科学技术发展的需要，七五期间中国科学院在上海细胞生物学研究所筹建中国科学院细胞库，并于1991年经中科院验收后正式启用。1996年中国科学院典型培养物保藏委员会成立，细胞库为其成员之一。2000年该库参加世界培养物保藏联合会，成为其登记成员之一。2002年挂靠在中科院上海生科院，名称为中国科学院上海生命科学研究院细胞资源中心。2013年，中科院细胞库回归中科院生化细胞所。细胞库原有各类细胞三百多种，面向全国常年提供各类细胞系。
中国科学院干细胞库成立于2007年1月，是科技部国家重大科学研究计划专项经费两次支持下（2007-2012年和2013-2017年）在全国范围内深度建设的四个干细胞库之一。中科院干细胞库依托中科院生化细胞所，迄今已收集保存了一百多种各类ES细胞、iPS细胞、成体干细胞、间充质干细胞和其它细胞系，总库容达八千余株。其技术人员在干细胞建系、保种、培养、鉴定、检测、诱导分化及基因敲除小鼠制备等方面具有丰富的经验，发展和完善了干细胞研究的关键技术平台，积累了不少科研成果并发表了一系列研究论文。目前，该库每年向国内上百家单位提供各种干细胞及各类技术服务，并常年提供干细胞相关技术咨询，有力地支持了全国干细胞研究的开展。
2014年初，中国科学院细胞库与干细胞库整合后形成新的细胞资源库，隶属于中科院生化细胞所，下属细胞库和干细胞库两个部门。目前，中国科学院细胞库已完成所有细胞资源的规范化和数字化整理，有四百多种细胞可对外提供资源共享服务，几乎涵盖了当前细胞生物学研究领域的所有细胞种类，是全国范围内细胞种类最全、供应量最大的资源中心之一。
中国科学院细胞库旨在收集、开发以及保藏我国和世界的人和动物的细胞株和干细胞(系)资源；研究和发展细胞培养新技术，研究和发展细胞株(系)的保藏、鉴定、质量控制和分发的新技术；面向全国，为我国生命科学和生物技术领域的研究工作和产业化提供标准化的细胞株(系)及相关技术服务。中国科学院细胞库
中国科学院上海生命科学研究院细胞资源中心
如要细胞,请先电话联系咨询有关细胞的背景和检测结果
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电话：021-54920404， 54920405
Fax: 021-54920406
联系人：吴军
e-mail: wjun@sibcb.ac.cn；cellcell@sibcb.ac.cn；cellbank@sibcb.ac.cn
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