外泌体计数、粒径、蛋白表达、蛋白共定位一次完成

实验原理

① 35 μL外泌体样品滴加在芯片上孵育；
② 预先包被的抗体特异结合外泌体表面蛋白以捕获外泌体；
③ 再使用荧光抗体特异性标记需要表征的标记物；
④ 最后用ExoView R100检测外泌体粒径、计数、蛋白表达（CD9，CD81，CD63等）及共定位。

检测流程

产品类别

试剂盒特点

外泌体荧光数量统计

外泌体粒径检测

外泌体内容物检测

荧光强度与粒径关系

荧光共定位分析

发表文章

• Andras Saftics.(2021) Data evaluation for surface-sensitive label-free methods to obtain real-time kinetic and structural information of thin films: A practical review with related software packages. Advances in Colloid and Interface Science. 
• Kyoung-Won Ko.(2021) Integrated Bioactive Scaffold with Polydeoxyribonucleotide and Stem-Cell-Derived Extracellular Vesicles for Kidney Regeneration. ACS Nano. 
• Tanina Arab. (2021) Characterization of extracellular vesicles and synthetic nanoparticles with four orthogonal single‐particle analysis platforms. Journal of Extracellular Vesicles. 
• Niaz Z.Khan.(2021) Spinal cord injury alters microRNA and CD81+ exosome levels in plasma extracellular nanoparticles with neuroinflammatory potential. Brain, Behavior, and Immunity. 
• Dario Brambilla. (2021) EV Separation: Release of Intact Extracellular Vesicles Immunocaptured on Magnetic Particles. Analytical Chemistry. 
• Enkhtuya Radna. (2021) Extracellular vesicle mediated feto-maternal HMGB1 signaling induces preterm birth. Lab on a Chip. 
• Li, M., Soder. (2021) WJMSC‐derived small extracellular vesicle enhance T cell suppression through PD‐L1. Journal of Extracellular Vesicles. 
• Crescitelli, R. (2021) Isolation and characterization of extracellular vesicle subpopulations from tissues. Nature protocols. 
• Berger, A. (2021). Local administration of stem cell-derived extracellular vesicles in a thermoresponsive hydrogel promotes a pro-healing effect in a rat model of colo-cutaneous post-surgical fistula. Nanoscale. 
• Vidal, M. (2020) Exosomes and GPI-anchored proteins: Judicious pairs for investigating biomarkers from body fluids. Advanced drug delivery reviews. 
• K Cho, H Kook.(2020)Study of immune-tolerized cell lines and extracellular vesicles inductive environment promoting continuous expression and secretion of HLA-G from semiallograft immune tolerance during pregnancy. Journal of Extracellular Vesicles. 
• Maximillian A. Rogers.(2020)Annexin A1–dependent tethering promotes extracellular vesicle aggregation revealed with single–extracellular vesicle analysis. Cell Biology. 
• Annette M. Marleau.(2020)Targeting tumor-derived exosomes using a lectin affinity hemofiltration device. Cancer Research. 
• Alessandro Gori.(2020)Membrane-Binding Peptides for Extracellular Vesicles On-Chip Analysis. Journal of Extracellular Vesicles. 
• Rossella Crescitelli.(2020)Subpopulations of extracellular vesicles from human metastatic melanoma tissue identified by quantitative proteomics after optimized isolation. Journal of Extracellular Vesicles. 
• Maria S. Panagopoulou.(2020) Phenotypic analysis of extracellular vesicles:  a review on the applications of fluorescence. Journal of Extracellular Vesicles.
• WeiYan.(2020) Immune Cell-Derived Exosomes in the Cancer-Immunity Cycle. Trends in Cancer. 
• Daniel Bachurski. (2019) Small RNA Sequencing across Diverse Biofluids Identifies Optimal Methods for exRNA Isolation. Cell.

用户单位

      Quantum Design中国子公司成立于2004年，拥有一支具备强大技术背景、职业化工作作风的团队，在全权负责美国Quantum Design公司本部产品在中国销售和售后服务的同时，公司作为Quantum Design全球代理分销网的重要成员，还积极致力于发展与其他先进科学仪器制造商的合作，帮助其将产品迅速引进中国市场、发展与中国本地科学家的合作将实验方法及设备商业化。Quantum Design中国子公司的长期目标是使自身最终成为中国与世界进行先进技术及仪器交流的一个重要桥头堡。目前，Quantum Design中国子公司正立足于公司本部产品，积极致力于材料物理、纳米表征和测量技术、生物及生命科学技术领域的新业务。 

      Quantum Design中国 生命科学事业部 致力于引进和推介生物领域内先进设备及技术。目前已与瑞士Cytosurge公司、美国NanoView公司、波兰Novilet公司、法国Abbelight公司、法国Telight公司、德国LLS ROWIAK公司、西班牙Planelight公司、美国Delong公司等十几家生物及医药仪器设备制造商建立合作关系，帮助中国市场引进更多全球范围内的优秀设备和技术，包含但不限于新一代研发的 多功能单细胞显微操作系统、小动物自由基成像系统、全自动外泌体荧光检测分析系统、光片显微镜、3D单分子荧光成像系统、超分辨共聚焦显微系统、原位细胞3D切割成像平台、3D组织切割成像系统、低电压台式透射电子显微镜、实时无标记细胞动态分析仪等，在生命科学领域的深度探索中，助力中国科学家的项目研究和发展。