荧光定量PCR技术服务
服务详情
荧光定量PCR服务要求
1. 请您提供新鲜的且尽量多的材料（各种材料的 RNA 提取量请参照“总 RNA 的提取”），或直接提供纯化好的 总 RNA （大于 5 ug/ 样品）；（ 各种材料的 DNA 提取量请参照“ D NA 的提取”），或直接提供纯化好的 D NA （大于 5 ug/ 样品 ) 。
2. 请通过 E-mail 提供已知的全长基因序列。
3. 请提供尽可能详细的背景资料： DNA/ RNA 来源、丰度等
荧光定量PCR操作程序
1. 根据基因序列设计特异性的引物和荧光探针。
2. 提取 DNA/ RNA 。
3. Real Time PCR(荧光定量PCR)，进行实验结果分析
4. 实验完成后，提供完整的实验报告（含软件分析结果）及引物等实验材料
荧光定量PCR收费标准
1、RNA提取：10－20个样品的RNA提取是：150元/个；20－30个样品的RNA提取是：120 元/个；30个以上样品的RNA提取是：100元/个；每份材料最少应提供：100mg/样品
2、一个指标每个样品为150元；二个指标每个样品为240元；三指标每个样品为300元，三个指标以上每个样品为80元/反应。
3、引物合成为每个碱基2.1元，探针标记为1200元/条，如果是用sybr green I(染料)做，则免去探针的费用，染料赠送。
4、内参的GAPDH引物探针免费，标准曲线免费赠送。
5、不再收取任何的基础费或者开机费。
(如果样本或者指标数更多，再面议)，5000.00元以内的服务费，一律100%预付；
公司简介
上海启发生物科技有限公司自2003年成立以来，始终致力面向生命科学领域，从事科研机构及生产企业所需的各类生产原料、试剂、仪器、耗材等的专业进出口公司。自成立以来发展迅速，产品服务得到全国广大生产企业、高校、科研院所、医疗系统及相关试剂公司的肯定，长期合作的客户遍及全国各地。并拥有自营进出口权，为更好地服务广大用户，不断降低生产成本并向我们的客户提供更强大的产品服务，同时为了将启发生物的质量管理提升到国际水平，启发生物正在申请ISO9001国际质量认证并已进入后期试运行阶段。我们愿竭尽所能，与广大生命科学工作者携手迎接属于生命科学的21世纪，共同创造新世纪的生物技术革命。
启发生物——让您重新审视您的供应商！
启发生物－－拥有美国采购中心的生物超市！超多品牌、超低批发价格！
启发生物——超强能力购买冷、偏、奇产品，Pierce、血清、Chemicon、Pharmacia大量现货！
重点品牌
RNA 干扰 Genetools 、Chemicon 、 Santa Cruz
信号转导研究 Genetools 、Chemicon 、CST 、Santa Cruz
分子生物学 Toyobo 、Promega 、Strategene、Bio-Rad
干细胞、细胞凋亡 Chemicon 、R&D 、Santa Cruz 、Biovision
细胞因子及检测 Chemicon 、R&D 、Peprotech 、Prospec
抗体与标记抗体 Santa Cruz 、 R&D 、 Abnova、Cayman
细胞培养及药筛 Clark、Cisbio、Hyclone 、Gibco、Chemicon
PCR与定量PCR Bio-Rad 、 Stratagene 、Promega 、Toyobo
蛋白质研究 Pierce 、 Pharmacia 、 Pannomics 、 Bio-Rad
A&G A-Msystems ABBOMAX ABCAM AbD Serotec ABEL Abfontier AbFrontier ABgene Abgent ABI Abnova ABR ACC Acrometrix Adipobiotech AdipoGen ADL Advanced Advanced Biotech Advanced Biotechnologies Incorporated ADVANTEC Advtech Affymetrix Agabead AGDIA Agilent Agrisera Airliquide AIRTECH airwell AKTA ALC Alcan Packaging ALCBIO Aldrich Alexis Allele-In-One Alleleustrious Alliance Alomone Alpha Innotech Alpha Diagnoestic International Amaxa Ambiogen Ambion Ameiriaia American Diagnostica Inc American Research Products Amersco Amersham Aminex Amresco AMS Anaspec Ancell ANGEL ANGENL Anigen anilab ANTEC anthos Antibodies AntibodyShop Antigenix Apogene Applichem Applied Imaging Apricot Arista Biologicals ARTmoderne Labortechnik AS ASSAY Assay Designs Assay Designs/Stressgen AssayMax ATCC ATGen AutoGen AvantGene AVESTIN Avidity AVIVA AXGEN Axis-Shield AXXORA Axygen
B-Bridge B.D. Bacou-Dalloz BANGS LAB Bangs Laboratories Bayer BD BDH BDIS BECKMAN Beckman Coulter BEIMEI BEMEI Bender Bender Medsystems BEOCO Berthold BERTHOLD Detection Systems BetaBasic Bethyl Beyotime BHM bio Bio-mark Bio-Rad bio-smark BIO-TEK Biochain Biochrom BIOCOAT biococ Biocolor biocomet biocomma biocytex Biodesign BIODESIGN BIODETECT Biodot bioeasy Bioer BIOFIL Bioflux BioFX Biohit BioInd BioLabs biolead BioLegend Biolife biological industries Biomat BIOMED BIOMOL Bioplastics Bioscora biosea Biosearch Technologies BIOSEC biosensis biosis Biosite BIOSOURCE Biospec Biospherical Bioss Biosystem bioteke Biothema Biotium Biotrin Biousing biov biovendor BioVision Biowest Biowhittaker bioworld Biozyme Bipec BLS BLYQ BMA BMBIO boatman BoMei Bonetrap 5b Boster boyun bpt Brady Brand BRT BTI BTX bushnell bxbio BYLAB Cadenza Caidon CAKON Calbiochem Caltag Cambion cambrex Can Get Signal CANAG capitalbio CAPP Cartesian Cayman CBI CBIO Cell Cell BioLabs Cell Signal Cell Signaling cellbank CellGro cellstar Cellufine centrebio CHANGCUN chemagic CHEMICON Chempette CHENXI CHIRALCEL Chondrex CHROMOGENIX Chromosorb chsd CIS CJC Clare Chemical Research CLEARVIEW ClonaCell Clontech CLONTECH Laboratories CLP CMA Cole-Parmer coleparmer Colitag CombiMatrix Comsobrand Contros copharin Corbet Cordlife Corning Cosmobrand Costar coulter COVANCE CP CRP Cryogenic Vial Holder Cryologic CST CYAGEN Cyagen Biosciences Cybio Cyclex Cygnus CytoBuoy Cytomark Cytoskeleton CytoSub CytoTrend 川崎 d&b D2650 Daicel Daiki DAISO DAKO DCS deconex DECOV Delta Biolabs deltaT DENDRITICS SAS Devatal Develosil DeVID DF-121 DGSC Dharmacon DHMARCOM Diaclone Diagnostic Biosystem DIAION Diazyme Difco DIONEX Discovery DivBio Dojindo DOMNICK HUNTER DR.E Dragon Dragonmed DRAND dsaf DSL DU pont dualsystems Dynabeads Dynex EasySep eBioscience Echelon Biosciences ECM Elga ELGA LabWater EMS Endecotts Endogen Engreen ENVI-Florisil EPICENTRE epigentic epiquick Epitomics eppendorf EQUL ES-Cult ESCO Ettan Ewig Exalpha EXCEEDER ExCell Exiqon FabGennix Fermentas FERMENTEK Filter Finnpipette Finnzymes Fisher Fitzgerald Fluka Fluke Fluorotechnics fs fuji fulengen Galen GE GEA Gemini Gene Therapy Systems Genecore GENEMED genesis Genetech Genetex Genisphere GenScript Gentra GenWay Gibco Gilson GL Biochem GloboZymes GLS Goat GOLDSIGN gonotec gotuwang Green Spring greienr griener GroPep GSD GYAN gz H&H H&R Haematologic Haier HAISEN HAMILTON Hamilton Microlab Hankison harvard Hbt HD Biosciences HDA-GT12 HDB HDN HEROCHEM Heto HEZI HHDGH HHIJIAN Hidex HILTI Hiross hitsanns HJ HK HMAILTON Hoefer Holten Honeywell Hotgen Houbio HQ Bioscience HRBio HS HTL HX HY Hyclone Hycult Biotechnology Hypersil hz iami IBA-go ibidi IBS ICPBIO ICT IDS IDVET illustra Imgenex Immundiagnostik ImmunoHunt ImmunoStep Imstar IMTEC IMUBIND INALCO infinitesky Infors Ingeny Inno-Train Innogent innovaquartz Inotech Intergen iNtRON Invirion Invitrogen Invivogen InVivoScribe IQ IWAKI iwill JENA Jenway JET JETBIO JETBIOFIL Jinsite JKY jnbio jsboeng JTBaker JUCHHEIM Kapa KAPAK Kartell KBY KeyGEN keyu KG Kimax Kimbery-clar Klyster knxm KODAK KPL KRH Kromasil Kromat KT KUBOTA Kyowa L1210 LAB Lab Vision lab-legens Lab-Tek LABAIDE labguide Labnet LABORATORIOS CONDA Labpower Labsyatems LABVISION landlab LANZA Larodan LC Sciences LDK LEGENS Leica leja lengton LENGTON DSL Li-Cor Lichrosorb Lichrospher LifeHolder Linc-Bio Linco Line-Gene lnvitrogen Lonza LPBIO LR Lucigen Lucky LUPI M199 Mabtech MACHEREY-NAGEL MagExtractor -Plasmid- Marker gene MART Matrx MatTek MBI MBL MCAO MCT MDDB medica MeDiPro Medisafe Merck Mercodia MERK MethoCult Mettler-Toledo MettlerToledo MG2 MI Micro-Guard mikrocount Millipore Millipre Miltenyi Biotec Miltenyibiotec Mini-instruments MiniStar Plus MiraiBio miRIDIAN Mirus MITSUBISHI MJ Research MK3 MKM MMM MN Molecular MOLTOX MONOSAN moor MOTIVE Mr. Frosty MRC MT MYCRO Nacalai NALENENE nalgene NanoBac naturegene NBD NEB Neomarker NeoMarkers NeuroCult Neuromics newcon NICHRYO nikon Nordic Norgen Biotek Novagen Novocastra nqbb Nunc Obelisc OEM OLYMPUS OMEGA Oncogene OPAS Open opti-logic Orange Orange Scientific Orbigen OriCell Origene ORION Orpegen osenses OSRAM otwo outdo OXIS Oxoid PALL palomare PANATecs Panomic Panomics Parafilm PARKER PBI PBL InterferonSource PC PE PeproTech PERI PerkinElmer pG PGMCHINA pgrp Phalanx Pharmacia pharmavan PHILIPS phoenix PIERCE pilotech PM Polyplus PosiTector UTG PPS Silent Primesep Progema PROGEN ProImmune Promega ProQinase prosci Proscientific Prospec ProSpec-Tany Proteintech Group Inc PROTERI Prozyme PTFE PTGlab PuriBest PX PYM PYRAMID Qbiogene QcBio QD QED QIAGEN QSP QuantumDot Quidel R&D r-biopharm Raybiotech RB RDH ReaConTM RealGene RECI recombinant Relia Tech Rezex RG RKP-POTASSIUM RND Robbins Scientific robiot ROCA Roche Rockland roloo ROSCO rpeptide Ruibio rwd RY SAB Sac-Cel saibio saierbio SAINT-GOBAIN SAKURA Sani-Tech sanlab Sanquin SANTA Santa Cruz Sanyo Sarstedet sartoris SARTORIUS Savvycheck SBA SBI Schleicher&Schuell SCHOTT science Sciencell scienhome Scientific Industries SCPS SD searchbio SEBC Seipettor SeitzSchenk/PALL SEPABEADS Sephadex Serocp SEROMP Serotec SERVA SH1OA SHELLAB Shelton shengyou SHIMADZU SHINE SCIENCE ShineGene SHINESO SHUNIU SIAL sigma Signosis simga sinogene SIPI-MRNG SKK\NSBP sms Socorex Sofarbio Softron solomonbio Sony SPB Spectro SPEX SPINREACT SPR Spring Bioscience SRK SSI Stanta Cruz Stemcell Stratagene Stressgen SUKUN Sunbio Sunbioi sunbiotech sunny Sunub SUPELCO SUPELCOSIL SUPELCOWAX Superarray Superspher sutter SVR SY-LAB SYNBEADS Synbiotics SYNCHEM Syngene TAMASE TBD TCL TEDIA thermo Thermo Scientific Ti20 TIANBO TIANGEN TK TKM TMC toboyo Tocris TOMOS Tomy TopoGen TOSOH touching TOYO ADVANTEC TOYOBO TPI TPP transglobal Treff TREK SENSITITRE Trinity Trizol TSE TSH TSK TYGON U-CyTech ub UCL Ultrafilter ULTRON ES-OVM UniQ UPSTATE usbio USCNLIFE UVP Vacuubrand Van Varian Vector VIAGENE Vibratome VideoTesT vigorous VILBER VIORION VIRION-SERION ViroStat Vistalab VITLAB VMRD VWR Vysis V_gene Wako WALZ WANGT waston Waters WEALTEC Wescor Whatman Wheaton Wielab Wieslab wolwobiotech Worthington WPA XB Xenopore XH xhyq xiangyi Yacoo YaKult.Co YAMATO YDI YHA YMC YRBIO zeiss ZeptoMetrix Zeus ZHICHENG ZJ Zymed Zymo Research
启发生物——让您重新审视您的供应商！
启发生物－－拥有美国采购中心的生物超市！超多品牌、超低批发价格！
启发生物——超强能力购买冷、偏、奇产品，Pierce、血清、Chemicon、Pharmacia大量现货！
重点品牌
RNA 干扰 Genetools 、Chemicon 、 Santa Cruz
信号转导研究 Genetools 、Chemicon 、CST 、Santa Cruz
分子生物学 Toyobo 、Promega 、Strategene、Bio-Rad
干细胞、细胞凋亡 Chemicon 、R&D 、Santa Cruz 、Biovision
细胞因子及检测 Chemicon 、R&D 、Peprotech 、Prospec
抗体与标记抗体 Santa Cruz 、 R&D 、 Abnova、Cayman
细胞培养及药筛 Clark、Cisbio、Hyclone 、Gibco、Chemicon
PCR与定量PCR Bio-Rad 、 Stratagene 、Promega 、Toyobo
蛋白质研究 Pierce 、 Pharmacia 、 Pannomics 、 Bio-Rad
A&G A-Msystems ABBOMAX ABCAM AbD Serotec ABEL Abfontier AbFrontier ABgene Abgent ABI Abnova ABR ACC Acrometrix Adipobiotech AdipoGen ADL Advanced Advanced Biotech Advanced Biotechnologies Incorporated ADVANTEC Advtech Affymetrix Agabead AGDIA Agilent Agrisera Airliquide AIRTECH airwell AKTA ALC Alcan Packaging ALCBIO Aldrich Alexis Allele-In-One Alleleustrious Alliance Alomone Alpha Innotech Alpha Diagnoestic International Amaxa Ambiogen Ambion Ameiriaia American Diagnostica Inc American Research Products Amersco Amersham Aminex Amresco AMS Anaspec Ancell ANGEL ANGENL Anigen anilab ANTEC anthos Antibodies AntibodyShop Antigenix Apogene Applichem Applied Imaging Apricot Arista Biologicals ARTmoderne Labortechnik AS ASSAY Assay Designs Assay Designs/Stressgen AssayMax ATCC ATGen AutoGen AvantGene AVESTIN Avidity AVIVA AXGEN Axis-Shield AXXORA Axygen
B-Bridge B.D. Bacou-Dalloz BANGS LAB Bangs Laboratories Bayer BD BDH BDIS BECKMAN Beckman Coulter BEIMEI BEMEI Bender Bender Medsystems BEOCO Berthold BERTHOLD Detection Systems BetaBasic Bethyl Beyotime BHM bio Bio-mark Bio-Rad bio-smark BIO-TEK Biochain Biochrom BIOCOAT biococ Biocolor biocomet biocomma biocytex Biodesign BIODESIGN BIODETECT Biodot bioeasy Bioer BIOFIL Bioflux BioFX Biohit BioInd BioLabs biolead BioLegend Biolife biological industries Biomat BIOMED BIOMOL Bioplastics Bioscora biosea Biosearch Technologies BIOSEC biosensis biosis Biosite BIOSOURCE Biospec Biospherical Bioss Biosystem bioteke Biothema Biotium Biotrin Biousing biov biovendor BioVision Biowest Biowhittaker bioworld Biozyme Bipec BLS BLYQ BMA BMBIO boatman BoMei Bonetrap 5b Boster boyun bpt Brady Brand BRT BTI BTX bushnell bxbio BYLAB Cadenza Caidon CAKON Calbiochem Caltag Cambion cambrex Can Get Signal CANAG capitalbio CAPP Cartesian Cayman CBI CBIO Cell Cell BioLabs Cell Signal Cell Signaling cellbank CellGro cellstar Cellufine centrebio CHANGCUN chemagic CHEMICON Chempette CHENXI CHIRALCEL Chondrex CHROMOGENIX Chromosorb chsd CIS CJC Clare Chemical Research CLEARVIEW ClonaCell Clontech CLONTECH Laboratories CLP CMA Cole-Parmer coleparmer Colitag CombiMatrix Comsobrand Contros copharin Corbet Cordlife Corning Cosmobrand Costar coulter COVANCE CP CRP Cryogenic Vial Holder Cryologic CST CYAGEN Cyagen Biosciences Cybio Cyclex Cygnus CytoBuoy Cytomark Cytoskeleton CytoSub CytoTrend 川崎 d&b D2650 Daicel Daiki DAISO DAKO DCS deconex DECOV Delta Biolabs deltaT DENDRITICS SAS Devatal Develosil DeVID DF-121 DGSC Dharmacon DHMARCOM Diaclone Diagnostic Biosystem DIAION Diazyme Difco DIONEX Discovery DivBio Dojindo DOMNICK HUNTER DR.E Dragon Dragonmed DRAND dsaf DSL DU pont dualsystems Dynabeads Dynex EasySep eBioscience Echelon Biosciences ECM Elga ELGA LabWater EMS Endecotts Endogen Engreen ENVI-Florisil EPICENTRE epigentic epiquick Epitomics eppendorf EQUL ES-Cult ESCO Ettan Ewig Exalpha EXCEEDER ExCell Exiqon FabGennix Fermentas FERMENTEK Filter Finnpipette Finnzymes Fisher Fitzgerald Fluka Fluke Fluorotechnics fs fuji fulengen Galen GE GEA Gemini Gene Therapy Systems Genecore GENEMED genesis Genetech Genetex Genisphere GenScript Gentra GenWay Gibco Gilson GL Biochem GloboZymes GLS Goat GOLDSIGN gonotec gotuwang Green Spring greienr griener GroPep GSD GYAN gz H&H H&R Haematologic Haier HAISEN HAMILTON Hamilton Microlab Hankison harvard Hbt HD Biosciences HDA-GT12 HDB HDN HEROCHEM Heto HEZI HHDGH HHIJIAN Hidex HILTI Hiross hitsanns HJ HK HMAILTON Hoefer Holten Honeywell Hotgen Houbio HQ Bioscience HRBio HS HTL HX HY Hyclone Hycult Biotechnology Hypersil hz iami IBA-go ibidi IBS ICPBIO ICT IDS IDVET illustra Imgenex Immundiagnostik ImmunoHunt ImmunoStep Imstar IMTEC IMUBIND INALCO infinitesky Infors Ingeny Inno-Train Innogent innovaquartz Inotech Intergen iNtRON Invirion Invitrogen Invivogen InVivoScribe IQ IWAKI iwill JENA Jenway JET JETBIO JETBIOFIL Jinsite JKY jnbio jsboeng JTBaker JUCHHEIM Kapa KAPAK Kartell KBY KeyGEN keyu KG Kimax Kimbery-clar Klyster knxm KODAK KPL KRH Kromasil Kromat KT KUBOTA Kyowa L1210 LAB Lab Vision lab-legens Lab-Tek LABAIDE labguide Labnet LABORATORIOS CONDA Labpower Labsyatems LABVISION landlab LANZA Larodan LC Sciences LDK LEGENS Leica leja lengton LENGTON DSL Li-Cor Lichrosorb Lichrospher LifeHolder Linc-Bio Linco Line-Gene lnvitrogen Lonza LPBIO LR Lucigen Lucky LUPI M199 Mabtech MACHEREY-NAGEL MagExtractor -Plasmid- Marker gene MART Matrx MatTek MBI MBL MCAO MCT MDDB medica MeDiPro Medisafe Merck Mercodia MERK MethoCult Mettler-Toledo MettlerToledo MG2 MI Micro-Guard mikrocount Millipore Millipre Miltenyi Biotec Miltenyibiotec Mini-instruments MiniStar Plus MiraiBio miRIDIAN Mirus MITSUBISHI MJ Research MK3 MKM MMM MN Molecular MOLTOX MONOSAN moor MOTIVE Mr. Frosty MRC MT MYCRO Nacalai NALENENE nalgene NanoBac naturegene NBD NEB Neomarker NeoMarkers NeuroCult Neuromics newcon NICHRYO nikon Nordic Norgen Biotek Novagen Novocastra nqbb Nunc Obelisc OEM OLYMPUS OMEGA Oncogene OPAS Open opti-logic Orange Orange Scientific Orbigen OriCell Origene ORION Orpegen osenses OSRAM otwo outdo OXIS Oxoid PALL palomare PANATecs Panomic Panomics Parafilm PARKER PBI PBL InterferonSource PC PE PeproTech PERI PerkinElmer pG PGMCHINA pgrp Phalanx Pharmacia pharmavan PHILIPS phoenix PIERCE pilotech PM Polyplus PosiTector UTG PPS Silent Primesep Progema PROGEN ProImmune Promega ProQinase prosci Proscientific Prospec ProSpec-Tany Proteintech Group Inc PROTERI Prozyme PTFE PTGlab PuriBest PX PYM PYRAMID Qbiogene QcBio QD QED QIAGEN QSP QuantumDot Quidel R&D r-biopharm Raybiotech RB RDH ReaConTM RealGene RECI recombinant Relia Tech Rezex RG RKP-POTASSIUM RND Robbins Scientific robiot ROCA Roche Rockland roloo ROSCO rpeptide Ruibio rwd RY SAB Sac-Cel saibio saierbio SAINT-GOBAIN SAKURA Sani-Tech sanlab Sanquin SANTA Santa Cruz Sanyo Sarstedet sartoris SARTORIUS Savvycheck SBA SBI Schleicher&Schuell SCHOTT science Sciencell scienhome Scientific Industries SCPS SD searchbio SEBC Seipettor SeitzSchenk/PALL SEPABEADS Sephadex Serocp SEROMP Serotec SERVA SH1OA SHELLAB Shelton shengyou SHIMADZU SHINE SCIENCE ShineGene SHINESO SHUNIU SIAL sigma Signosis simga sinogene SIPI-MRNG SKK\NSBP sms Socorex Sofarbio Softron solomonbio Sony SPB Spectro SPEX SPINREACT SPR Spring Bioscience SRK SSI Stanta Cruz Stemcell Stratagene Stressgen SUKUN Sunbio Sunbioi sunbiotech sunny Sunub SUPELCO SUPELCOSIL SUPELCOWAX Superarray Superspher sutter SVR SY-LAB SYNBEADS Synbiotics SYNCHEM Syngene TAMASE TBD TCL TEDIA thermo Thermo Scientific Ti20 TIANBO TIANGEN TK TKM TMC toboyo Tocris TOMOS Tomy TopoGen TOSOH touching TOYO ADVANTEC TOYOBO TPI TPP transglobal Treff TREK SENSITITRE Trinity Trizol TSE TSH TSK TYGON U-CyTech ub UCL Ultrafilter ULTRON ES-OVM UniQ UPSTATE usbio USCNLIFE UVP Vacuubrand Van Varian Vector VIAGENE Vibratome VideoTesT vigorous VILBER VIORION VIRION-SERION ViroStat Vistalab VITLAB VMRD VWR Vysis V_gene Wako WALZ WANGT waston Waters WEALTEC Wescor Whatman Wheaton Wielab Wieslab wolwobiotech Worthington WPA XB Xenopore XH xhyq xiangyi Yacoo YaKult.Co YAMATO YDI YHA YMC YRBIO zeiss ZeptoMetrix Zeus ZHICHENG ZJ Zymed Zymo Research
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