其他生物研发

Stable Cell Line Services

基本信息
服务名称:
Stable Cell Line Services
英文名称:
Stable Cell Line Services
参考报价:
总点击数:
2175
更新日期:
2012-04-23
服务类别:

服务详情

 

Development of a stable cell line based on CHO or NS0 for production of recombinant proteins and monoclonal antibodies is unavoidable for some applications such as clinical use, when mammalian cell expression system is pursued. However, stable cell line generation is mostly thought as time and cost-consuming work.

ACRObiosystems' provide commercial and research CHO(DG44, DUKX-B11, CHO-S or CHO-K1) cell line based on its proprietary expression vector, high throughput cell screening technologies and serum,animal components free process without any adaptation issue in a significantly reduced timeline

Service Specification


Service Content Approaches  Deliverables Timeline

Gene synthesizing
&Plasmid construction

  • In house developed codon optimization
  • Gene synthesizing and verification(in house)
  • Clone to proprietary mammalian expression vector
  • Gene sequencing report
  • Constructed plasmid
25days
Transient expression evaluation
  • Shake flaks scale transient transfection
  • Elisa based expression level measurement 
  • Expression feasibility
10days
Transfection and clone screening
  • 3 approaches, 3 rounds of clone selection
  • 5 top clones expand to shake flask scale for evaluation
  • 50 PDL stability evaluation upon request(additional time needed)
  • Three top clones
  • Expression stability data
  • Sterility/mycomplasma test
  • Other QC test upon request
40-60days
Process Development(optional)
  • Mutiple in-house developed chemically defined media/feeds platform screening and optimization
  • High throughput purification processes screening via DoE
  • Analytical assay development
  • Milligram to gram purified proteins
  • Process report
Quote
Production Service(optional)
  • 10L-50L bioreactor run
  • Chemically defined medium/feeds
  • Process control
  • Purified proteins
  • Process profile
  • Production report and other documentation upon request
Quote

Quotations and Ordering

You can place the order on line and make the payment with credit card or Paypal. Also you can place the order off-line by phone, email, fax with a formal PO (Purchase Order) or credit card. We recommend that you submit your service requests and orders on line. 

For questions and quotation requests, please submit your on-line request. Our customer specialist will get back to you in 24 hours from Monday through Friday. However, you can also contact us by email, phone, or fax.

Email: order@acrobiosystems.com
Phone: +1 301-825-5518(US)   +44 20-8123-6518(EU)   +86 400-685-2052(AP)
Fax: +1 301-500-2214

 

公司简介
ACROBiosystems是一家致力于肿瘤免疫治疗领域,专门从事重组蛋白开发生产的高新企业。其产品和服务面向全球制药公司、生物技术公司及科研院所,为抗体药物研发从上游到下游提供全方位整体解决方案。同时还为细胞治疗行业提供高品质的细胞因子、培养基等产品。
 
ACROBiosystems具有多系统蛋白表达能力,目前最具优势的是HEK Max人源细胞高效蛋白表达平台,除此之外还有大肠杆菌、酵母、昆虫细胞等表达系统。不仅如此,重点蛋白产品还具有多种属、多标签可供选择。其中最具特色的生物素标记蛋白获得了海内外用户的一致认可。
 
ACROBiosystems有着严格的质量控制体系及完善的客服流程,以确保客户满意度及产品认可度为宗旨。目前的业务已经遍及全球50多个国家和地区,并在北美、亚洲、欧洲等不同地区设立分支机构以便服务当地客户。
 
目前ACROBiosystems最具特色的产品系列有:
生物素标记蛋白(Biotinylated Proteins)
免疫检查点蛋白(Checkpoint Proteins)
Fc受体蛋白(Fc Receptor Family Proteins)
肿瘤药物靶点蛋白(Drug Target Proteins)
PD-1 [Biotinylated] : PD-L1抑制剂筛选ELISA试剂盒(PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair)
5-min单克隆抗体分型试剂盒(Five-Minute Mouse/Rat Isotyping Kit )
细胞因子、培养基、纯化介质及核酸酶



 


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详细地址:
北京经济技术开发区宏达北路8号5号楼4层
qq:
1109674318
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联系电话:

400-682-2521

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