其他生物研发

Mammalian Cells RPP Services

基本信息
服务名称:
Mammalian Cells RPP Services
英文名称:
Mammalian Cells RPP Services
参考报价:
总点击数:
1017
更新日期:
2012-04-23
服务类别:

服务详情

Mammalian cells have great advantages over other host systems on production of recombinant proteins,antibodies and some viral proteins in terms of the ability to get fully funtional proteins with properly folded structure, and complex N-linked and O-linked glycosylation.The comprehensive post-translational modification machinery enables reserachers to get funactional proteins for most applications. Compared with stable cell line, transient expression using CHO or 293 has got a lot of interests in recent years due to its short timeline, low cost and high quality.

ACRObiosystems' rapid protein production(RPP)services are based on transient gene expression using CHO and 293,we can deliver to customers grams level of recombinant proteins and monoclonal antibodies in a short timeline using our proprietary platforms.
* Risk-free trial for clients.Send DNA sequences via the request form, and we’ll take care of the rest.

Service Specification

Service Content Approaches  Deliverables Timeline

Gene synthesizing
&Palsmid construction

  • In-house developed codon optimization
  • Gene synthesizing and verification
  • Clone to proprietary mammalian expression vector
  • Gene sequencing report
  • Constructed plasmid
20 days
Plasmid preparation
  • E.coli fermentation
  • Large quantity of plasmid preparation using proprietary technology 
  • > 1mg low endotoxin plasmid DNA
2 days
Pilot evaluation of transient expression and purification
  • Mutiple in-house developed chemically defined media/feeds platform screening
  • High throughput purification processe screening via DoE
  • Tag free or removal considerations
  • Expression level
  • Purification process data
4-6 days
Process scale up and prodcution
  • Seeds train expansion,bioreactor run and clarification after harvest
  • Chromatography column purification
  • Analytical assay
  • Milligram to gram purified proteins with quality data
  • Documentation
8-10 days

Overlaped timeline: 2-3 weeks * Timeline of service is adjusted based on clients' requirements;

Quotations and Ordering

You can place the order on line and make the payment with credit card or Paypal. Also you can place the order off-line by phone, email, fax with a formal PO (Purchase Order) or credit card. We recommend that you submit your service requests and orders on line. 

For questions and quotation requests, please submit your on-line request. Our customer specialist will get back to you in 24 hours from Monday through Friday. However, you can also contact us by email, phone, or fax.

Email: order@acrobiosystems.com
Phone: +1 301-825-5518(US)   +44 20-8123-6518(EU)   +86 400-685-2052(AP)
Fax: +1 301-500-2214

公司简介
ACROBiosystems是一家致力于肿瘤免疫治疗领域,专门从事重组蛋白开发生产的高新企业。其产品和服务面向全球制药公司、生物技术公司及科研院所,为抗体药物研发从上游到下游提供全方位整体解决方案。同时还为细胞治疗行业提供高品质的细胞因子、培养基等产品。
 
ACROBiosystems具有多系统蛋白表达能力,目前最具优势的是HEK Max人源细胞高效蛋白表达平台,除此之外还有大肠杆菌、酵母、昆虫细胞等表达系统。不仅如此,重点蛋白产品还具有多种属、多标签可供选择。其中最具特色的生物素标记蛋白获得了海内外用户的一致认可。
 
ACROBiosystems有着严格的质量控制体系及完善的客服流程,以确保客户满意度及产品认可度为宗旨。目前的业务已经遍及全球50多个国家和地区,并在北美、亚洲、欧洲等不同地区设立分支机构以便服务当地客户。
 
目前ACROBiosystems最具特色的产品系列有:
生物素标记蛋白(Biotinylated Proteins)
免疫检查点蛋白(Checkpoint Proteins)
Fc受体蛋白(Fc Receptor Family Proteins)
肿瘤药物靶点蛋白(Drug Target Proteins)
PD-1 [Biotinylated] : PD-L1抑制剂筛选ELISA试剂盒(PD-1 [Biotinylated] : PD-L1 Inhibitor Screening ELISA Assay Pair)
5-min单克隆抗体分型试剂盒(Five-Minute Mouse/Rat Isotyping Kit )
细胞因子、培养基、纯化介质及核酸酶



 


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北京经济技术开发区宏达北路8号5号楼4层
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1109674318
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400-682-2521

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