采用Pippin系列全自动核酸电泳和片段回收系统文章1
下列发表的文章是采用美国Sage Science公司的Pippin Prep和Blue Pippin 全自动核酸电泳和片段回收系统或者采用其技术或材料
环亚生物科技有限公司(APG BIO Ltd)是美国Sage Science公司在中国大陆的总代理商,详情请登录www.apgbio.com
April 2013
Shining a Light on Dark Sequencing: Characterising Errors in Ion Torrent PGM Data
Authors:
Lauren M. Bragg, Glenn Stone, Margaret K. Butler, Philip Hugenholtz, Gene W. Tyson
Info:
The authors use the Pippin Prep as an alternative to gel purification for isolating PCR fragments. The authors note that manual gel purification can lead to underrepresentation of low G+C% sequences.
Citation:PLoS Comput Biol 9(4):e1003031
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April 2013
Transcriptome Analysis of Thapsia laciniata Rouy Provides Insights into Terpenoid Biosynthesis and Diversity in Apiaceae
Authors:
Damian Paul Drew, Bjørn Dueholm, Corinna Weitzel, Ye Zhang, Christoph W. Sensen, and Henrik Toft Simonsen
Info:
The Pippin Prep was used to size-selec Illumina RNA-seq libraries.
Citation:
Int. J. Mol. Sci. 2013, 14, 9080-9098
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April 2013
Selective Inhibition of Tumor Oncogenes by Disruption of Super-Enhancers
Authors:
Jakob Love, Heather A. Hoke, Charles Y. Lin, Ashley Lau, David A. Orlando, Christopher R. Vakoc, James E. Bradner, Tong Ihn Lee, and Richard A. Young
Info:
The Pippin Prep was used to size-selec ChIP-seq libraries.
Citation:Cell 153:320-334 April 2013
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March/April 2013
H2-Independent Growth of the Hydrogenotrophic Methanogen Methanococcus maripaludis
Authors:
Kyle C. Costa, Thomas J. Lie, Michael A. Jacobs, John A. Leigh
Info:The authors constructed deletions in several key metabolism genes to study the mechanism of H2 independent growth in this hydrogenotropic strain. Several mutants were found that shed new light on alternative mechanisms of methanogenic growth. The mutants were mapped by whole genome sequencing, in which Pippin Prep was used to size selec the libraries.
Citation:mBio (4)2:00062-13 March/April 2013
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January/February 2013
Draft Genome Sequence of Rhodococcus opacus Strain M213 Shows a Diverse Catabolic Potential
Authors:
Ashish Pathak, Stefan J. Green, Andrew Ogram, Ashvini Chauhana
Info:
Rhodococcus strains have diverse and unusual metabolic pathways that are being considered for industrial roles in bioproduction of chemicals and fuels. The authors present the whole genome sequence of one such strain. Nextera chemistry was used with size selection on the Pippin Prep to generate the sequencing libraries.
Citation:genomeA (1)1:e00144-12 January/February 2013
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January 2013
A genome-wide survey of genetic variation in gorillas using reduced representation sequencing
Authors:
Aylwyn Scally, Bryndis Yngvadottir, Yali Xue, Qasim Ayub, Richard Durbin, Chris Tyler-Smith
Info:
The study used reduced representation libraries to study diversity among the two currently recognized species of gorilla. The libraries were generated by AluI digestion followed by size selection on the Pippin Prep for AluI fragments between 150-250 bp.
Citation:pre-print
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January 2013
Use of microarray hybrid capture and next-generation sequencing to identify the anatomy of a transgene
Authors:
Amanda J. DuBose, Stephen T. Lichtenstein, Narisu Narisu, Lori L. Bonnycastle, Amy J. Swift, Peter S. Chines and Francis S. Collins
Info:
The authors designed a general method to study the arrangement of foreign DNA and host DNA rearrangements in trangenic animals. The protocol involves standard Illumina PE library generation, using the Pippin Prep for size selection, followed by microarray hybridization capture to isolate fragments containing the transgene.
Citation:
Nucleic Acids Research, 2013, 1-5
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January 2013
Discrete genetic modules are responsible for complex burrow evolution in Peromyscus mice
Authors:
Jesse N. Weber, Brant K. Peterson & Hopi E. Hoekstra
Info:
The work seeks to identify genetic influences on a complex behavior in mice, burrow formation. The authors used a modified RADseq procedure to genotype two species of wild mice with distinct burrowing behavior. Pippin Prepwas used for size selection in their RADseq protocol.
Citation:Letter, Nature 493:402-405
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环亚生物科技有限公司(APG BIO Ltd)成立于中国香港,专注于引进国外创新的技术和高品质的实验室仪器设备以及配套的试剂和耗材,为客户的实验提供优化的整体应用解决方案。
环亚生物科技有限公司(APG BIO Ltd)配备了专业的技术支持和应用支持,致力为用户提供完善的售前咨询和售后服务
环亚生物科技有限公司(APG BIO Ltd)在中国区总代理的进口产品有:
Ø 美国Aplegen公司的OmegaLum系列化学发光、荧光和可见光凝胶成像系统,
Ø 美国Precision Biosystems公司的BlotCycler全自动蛋白质印迹杂交系统
Ø 美国Sage Science公司的Pippin Prep,Blue Pippin全自动电泳和核酸片段回收系统,Pippin Pulse脉冲场电泳系统
Ø 瑞士Elchrom Scientific公司的Origins高分辨率电泳系统
Ø 美国MaestroGen公司的MaestroNano超微量紫外分光光度计,UltraSlim和UltraBright 无损伤蓝光切胶仪,BandPeeper实时凝胶电泳观察仪,MaestroSafe无毒核酸荧光染料
Ø 德国VWR公司的mySPEC系列超微量分光光度计
Ø 美国Advansta公司的WesternBright ECL发光液
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