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采用Pippin系列全自动核酸电泳和片段回收系统文章2

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采用Pippin系列全自动核酸电泳和片段回收系统文章2
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1583
更新日期:
2023-10-17
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 下列发表的文章采用美国Sage Science公司的Pippin Prep和Blue Pippin 全自动核酸电泳和片段回收系统或者从采用其技术或材料:

 环亚生物科技有限公司(APG BIO Ltd)是美国Sage Science公司在中国大陆的总代理商,详情请登录www.apgbio.com

August 2013

De novo transcriptome assembly of drought tolerant CAM plants, Agave deserti and Agave tequilana

Authors:
Stephen M Gross, Jeffrey A Martin, June Simpson, María Jazmín Abraham-Juarez, Zhong Wang and Axel Visel

Info:
Agave is an interesting plant for bioenergy study. This paper provides transcriptome data of two strains. The Pippin Prep was used to size selec cDNAs at 250 or 500 bp, followed by TrueSeq library construction and sequencing on the Illumina HiSeq.

Citation:BMC Genomics 2013 14:563
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August 2013

Comparative genomics of Campylobacter concisus isolates reveals genetic diversity and provides insights into disease association

Authors:
Nandan P Deshpande, Nadeem O Kaakoush, Marc R Wilkins and Hazel M Mitchell

Info:
Collaborators at the University of New South Wales sequence several strains of Campylobacter concisus from healthy and diseased (Crohn’s disease and gastroenteritis) individuals for a comparative study. Results showed genetice diversity and identified pathways that may be associated with the diseases. Pippin Prep was used to size selec libraries for Illumina HiSeq.

Citation:BMC Genomics 2013 14:585
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July 2013

Phylogeographic Origin of Helicobacter pylori Determines Host-Adaptive Responses upon Coculture with Gastric Epithelial Cells

Authors:
Alexander Sheh, Rupesh Chaturvedi, D. Scott Merrell, Pelayo Correa, Keith T. Wilson, James G. Fox

Info:
A study of gene expression comparing several strains of H. pylori. The BluePippin was used to size-selec fragments to construct paired-end libraries for Illumina MiSeq.

Citation:Infection and Immunity, July 2013 Volume 81 Number 7
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June 2013

The new sequencer on the block: comparison of Life Technology’s Proton sequencer to an Illumina HiSeq for whole-exome sequencing

Authors:
Joseph F. Boland, Charles C. Chung, David Roberson, Jason Mitchell, Xijun Zhang, Kate M. Im, Ji He, Stephen J. Chanock, Meredith Yeager, Michael Dean

Info:
An exome-sequencing study from the NIH comparing Illumina/Nimblegen to Ion Torrent/TargetSeq. Pippin size selection was used for library construction for Ion Proton sequencing.

Citation:Hum Genet (2013) 132:1153–1163
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June 2013

Skin Microbiome Imbalance in Patients with STAT1/STAT3 Defects Impairs Innate Host Defense Responses

Authors:
Sanne P. Smeekens, c Curtis Huttenhower, Anca Riza, Frank L. van de Veerdonk, Patrick L.J.M. Zeeuwen, Joost Schalkwijk, Jos W.M. van der Meer, Ramnik J. Xavier, Mihai G. Netea, Dirk Gevers

Info:
Genomic DNA was subjected to 16S PCR amplifications. Pippin Prep was used to retrieve the amplicons in order to isolate fragments away from aspecific products, prior to Illumina MiSeq sequencing.

Citation:J Innate Immun

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May 2013

Characterizing and measuring bias in sequence data

Authors:
Michael G Ross, Carsten Russ, Maura Costello, Andrew Hollinger, Niall J Lennon, Ryan Hegarty, Chad Nusbaum and David B Jaffe

Info:
Researchers at the Broad Institute compare sequence bias on Illumina, Ion, PacBio, and Complete Genomics platforms. Pippin sizing was used on the Ion Torrent libraries. The authors note that library construction conditions significantly influence sequencing bias.

Citation:Genome Biology 2013, 14:R51

公司简介

环亚生物科技有限公司(APG BIO Ltd)成立于中国香港,专注于引进国外创新的技术和高品质的实验室仪器设备以及配套的试剂和耗材,为客户的实验提供优化的整体应用解决方案。
    环亚生物科技有限公司(APG BIO Ltd)配备了专业的技术支持和应用支持,致力为用户提供完善的售前咨询和售后服务

 环亚生物科技有限公司(APG BIO Ltd)在中国区总代理的进口产品有:

Ø         美国Aplegen公司的OmegaLum系列化学发光、荧光和可见光凝胶成像系统,

Ø         美国Precision Biosystems公司的BlotCycler全自动蛋白质印迹杂交系统

Ø         美国Sage Science公司的Pippin Prep,Blue Pippin全自动电泳和核酸片段回收系统,Pippin Pulse脉冲场电泳系统

Ø         瑞士Elchrom Scientific公司的Origins高分辨率电泳系统

Ø         美国MaestroGen公司的MaestroNano超微量紫外分光光度计,UltraSlim和UltraBright 无损伤蓝光切胶仪,BandPeeper实时凝胶电泳观察仪,MaestroSafe无毒核酸荧光染料

Ø         德国VWR公司的mySPEC系列超微量分光光度计

Ø         美国Advansta公司的WesternBright ECL发光液


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