DNA-In® 293 Transfection Reagent

A Transfection Reagent Specifically for HEK293 Cells 

DNA-In® Cell-Specific Transfection Reagents are a line of transfection products developed by the lead members of the scientific team that invented and developed the Lipofectamine® family of reagents. These cell-specific reagents offer maximum DNA delivery in targeted common cell lines.  

The cell-specific DNA-In® 293 Transfection Reagent provides maximum high transfection, expression levels and cell viability in HEK293 cells. 

• Product sheet

• Features

• Fast, simple, easy-to-follow protocol

• One-step process: simply add DNA-In® 293 Transfection Reagent to diluted DNA, incubate 10 minutes, and add to pre-plated cells

• Minimal toxicity - no need to replace medium after transfection

DNA-In® 293 Transfection Reagent  Quick-Start Protocol

Superior Transfection Efficiency and Maximum Cell Viability

During its development and validation process, DNA-In® 293 Transfection Reagent underwent extensive testing in many primary and cultured cells.  In a head-to-head comparison study,  DNA-In® 293 Transfection Reagent consistently outperformed Lipofectamine® 2000 and Lipofectamine® 3000 transfection reagents. 

The following charts show side-by-side comparisons of DNA-In® 293 Transfection Reagent , Lipofectamine® 2000 (LF2K) and Lipofectamine® 3000 (LFK) in HEK293 cells:

DNA-In® 293 Transfection Reagent provides Maximum Transfection Efficiency in HEK293 cells

Above, HEK293 cells were plated at 70% confluency and transfected with the EF-1 alpha promoter-GFP 24 hours after plating. DNA-In® 293 transfected cells (top image) provides much improved transfection efficiency and cell viability as compared to LF3K transfected cells (bottom image).