在全世界生产的最重要的粮食作物中，有5-20%被昆虫吃掉。此外，与昆虫有关的病媒传播的疾病占所有传染病的17%以上，每年造成70多万人死亡和数百万例登革热、疟疾、锥虫病、莱姆病等等。这种破坏和疾病大部分是可以通过及时的干预和预防来避免的。

在物种水平上准确识别昆虫是很重要的，原因有很多，如指导杀虫剂控制措施，识别检疫性害虫，以及区分病媒昆虫等等。这方面的评估工作既要求准确性，同时也对通量有较高的要求。

当样品材料非常小且难以操作时，如昆虫的腿、幼虫和/或卵，DNA提取过程往往很复杂。从一个小样本中提取DNA通常会导致DNA产量和质量的巨大差异。

MicroGEM的prepGEM通用试剂盒提供快速和简单的单管提取，从新鲜或冷冻的完整或粉碎的昆虫和昆虫部位提取单链DNA。这种单管提取方法可以从非常小的样品中提取核酸，并确保样品中的所有核酸都被保存下来。

通过prepGEM通用试剂盒从各种昆虫样品中提取核酸的研究实例发表的文章如下：

1、农业：

研究表明，prepGEM有助于有害昆虫的检出以及潜在生物防治剂的鉴定。

Natural enemies of armored scales (Hemiptera: Diaspididae) and soft scales (Hemiptera: Coccidae) in Chile: Molecular and morphological identification
https://doi.org/10.1371/journal.pone.0205475

Investigating Biological Control Agents for Controlling Invasive Populations of the Mealybug Pseudococcus comstocki in France
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0157965

Detection of spotted-wing drosophila, Drosophila suzukii
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0098934

Detection of Pacific fruit fly, Bactrocera xanthodes
https://www.cambridge.org/core/journals/bulletin-of-entomological-research/article/dna-barcoding-and-realtime-pcr-detection-of-bactrocera-xanthodes-tephritidae-diptera-complex/82BF63629E12C3DEA22BAB5A7149FC46

Detection of African fig fly, Zaprionus indianus
http://www.bulletinofinsectology.org/pdfarticles/vol70-2017-057-062kremmer.pdf

Phylogenetic analysis of pest South Pacific sap beetle species
https://www.ncbi.nlm.nih.gov/pubmed/22613670

Characterising Aphelinid wasps as a biological control agent
https://link.springer.com/article/10.1007/s10526-016-9752-1

Characterising a Nematode species as a biological control agent
https://www.nzpps.org/journal/65/nzpp_651610.pdf

Identification of scale insects on kiwifruit in New Zealand
https://microgembio.com/wp-content/uploads/2019/10/MAPN005_20191002_Insect_prepGEM.pdf

2、病媒昆虫：

这些研究表明，prepGEM的提取适合于识别和监测病媒昆虫的研究。

Identification of clade types of malaria vector mosquito in north-eastern Tanzania
https://onlinelibrary.wiley.com/doi/full/10.1111/mve.12274

Monitoring insecticide resistance in malaria vector mosquito in south-east Tanzania
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5782413/

Understanding the population structure of a species of tsetse fly in Uganda
https://parasitesandvectors.biomedcentral.com/articles/10.1186/1756-3305-5-222#Sec2

3、实验室研究

一个常用的模型生物是线虫（秀丽隐杆线虫）。prepGEM已经被用来秀丽隐杆线虫的种群研究，重点是遗传多样性和繁殖。

Characterising male genotypes of C. elegans in response to a novel environment
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0035811#s3

Understanding genotype distribution during the experimental evolution of C. elegans
https://onlinelibrary.wiley.com/doi/full/10.1111/j.1558-5646.2012.01744.x

4、水产养殖

prepGEM已被用于水产养殖业，从岩龙虾幼体中提取DNA以确定生物种类。它还被用来开发一种低成本的从虾中提取DNA的方法，用于育种计划中的基因分型。

DNA barcoding of rock lobster species for feeding studies
https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0042757#abstract0

Developing a low-cost method for genotyping shrimp for breeding https://www.sciencedirect.com/science/article/abs/pii/S004484861400297X