概述
Introduction
纯羊乳是高端乳制品,脂肪球小、易吸收,适合乳糖不耐受者、老人和婴幼儿,还富含乳清蛋白、免疫球蛋白及多种营养素,不饱和脂肪酸占比高。
因其产量少、供需缺口大,发生多起羊乳掺假问题,主要以成本更低的牛乳为掺假成分。针对频发羊乳掺假问题,消费者面临牛乳过敏风险,可能引发皮肤瘙痒、红肿、恶心呕吐,甚至导致喉头水肿、呼吸困难、过敏性休克;企业会因产品不合格引发纠纷,造成经济损失、损坏品牌信誉。
青岛普瑞邦生物工程有限公司推出PriboStripTM羊乳中牛乳掺假快速检测试纸条,利用双抗夹心法原理,灵敏度为1 %,通过检测牛乳中的α-乳白蛋白初步判定羊乳中是否掺入牛乳,为食品生产及检测机构提供高效可靠的检测解决方案,助力食品安全。
Pure goat milk is a high-end dairy product. It contains small fat globules and is easily absorbed, making it suitable for people with lactose intolerance, the elderly, and infants. It is also rich in whey protein, immunoglobulins, and various nutrients, with a high proportion of unsaturated fatty acids.
Due to limited goat milk production, a significant gap exists between supply and demand. As a result, goat milk is sometimes adulterated with cow milk to reduce production costs. The frequent occurrence of such adulteration poses health risks to consumers, particularly those allergic to cow milk. Allergic reactions may include skin itching, redness and swelling, nausea, and vomiting, and in severe cases may lead to laryngeal edema, breathing difficulties, or anaphylactic shock. In addition, enterprises may face consumer disputes due to substandard products, resulting in economic losses and damage to brand reputation.

产品原理及优势
Product Principles and Advantages
双抗夹心法:利用抗α-乳白蛋白单克隆抗体 1(Ab-1)和抗体 2 与胶体金的结合物(Ab-2-Gdd)结合抗原的不同表位,从而实现对目标蛋白的定性检测。
1. 特异性强:采用抗α-乳白蛋白单克隆抗体 1,特异性识别牛乳中α-乳白蛋白,与其他常规基质无交叉反应。
2. 灵敏度高:灵敏度为1 %,每百毫升羊奶含1 mL牛奶(稀释100倍)时可检出阳性。
3. 操作便捷高效:样本前处理只需稀释即可直接检测,10 min内肉眼可直接判读结果。
4. 应用场景广泛:可用于羊乳加工厂的原料入厂质控、奶站、牧场及经销商的成品入库检验、以及监管部门产品品质抽查等场景。
Detection Principle
Bispecific antibody sandwich method: A conjugate of anti-α-lactalbumin monoclonal antibody 1 (Ab-1) and colloidal gold-labeled monoclonal antibody 2 (Ab-2-Au) is used to bind different epitopes of the target antigen. Through this specific antigen–antibody reaction, qualitative detection of the target protein is achieved.
Product Features
1)Strong specificity:
Anti-α-lactalbumin monoclonal antibody 1 specifically recognizes α-lactalbumin in cow’s milk and shows no cross-reactivity with other common food matrices.
2)High sensitivity:
The detection sensitivity is 1%. A positive result can be obtained when 1 mL of cow’s milk is present in 100 mL of goat milk (equivalent to a 1:100 dilution).
3)Convenient and efficient operation:
Sample pretreatment requires only simple dilution. Testing can be performed directly, and results can be read by the naked eye within 10 minutes.
4)Application scenarios:
Suitable for raw material quality control in goat milk processing plants, inspection of finished products at milk stations, pastures, and distribution points, as well as product quality spot checks by regulatory authorities.
产品参数
Product Parameter


检测方法
Method
1. 使用前将试纸条和待检样本溶液恢复至室温。
2. 将羊奶或羊奶粉用样本稀释液稀释100倍为待测液。
3. 取 200 uL 待测液加入离心管中,将取出的试纸条直接插入离心管中,注意液面高度勿高于箭头处。10min后即可判读结果。
1. Before use, allow the test strip and the sample solution to reach room temperature.
2. Dilute the goat milk or goat milk powder 100-fold with the sample diluent to obtain the test solution.
3. Pipette 200 µL of the test solution into a centrifuge tube. Insert the test strip directly into the tube. Ensure that the liquid level does not exceed the arrow mark on the strip. Interpret the result after 10 minutes.

检测结果
Results Analysis

1. 当试纸条仅有C线呈色时,为阴性结果。
2. 当试纸条C线与T线呈色时,为阳性结果。
3. 当试纸条C线不呈色时,为反应失败。请检查试纸条外包装是否破损、试纸条本身是否受潮,或样本浓度是否过高,并重新取一组试纸条做测试。
1. Negative result: Only the C line of the test strip is colored.
2. Positive result: Both C and T lines are colored.
3. Invalid result: When the C line of the test strip is not colored, the reaction fails. Please check the following possible reasons, and retest with a new test strip:
A: If the test strip packaging is damaged.
B: If the test strip is damp.
C: If the sample is too thick or too concentrated.
过敏原产品系列
Allergen product series
其他牛奶检测相关产品如下:
Other milk testing related products are as follows:


青岛普瑞邦PriboStrip™ 全系列食品过敏原快速检测平台提供多种的过敏原检测产品,助力企业严控过敏原交叉污染风险,具体项目如下:
• 八大过敏原类: 花生、大豆、鸡蛋、麸质、甲壳类、鱼类、牛奶、坚果(如杏仁、榛子)等。
• 其他常见过敏原类:芝麻、荞麦、苦荞、溶菌酶和组胺等。
• 蔬菜系列:羽扇豆、芹菜等。
• 调味品系列:芥末。
Qingdao PriboStrip™ offers a full range of rapid food allergen detection platforms, providing a wide variety of allergen detection products to help enterprises effectively control the risk of allergen cross-contamination. The available products include:
• The eight major allergens: peanuts, soybeans, eggs, gluten, crustaceans, fish, milk, nuts (such as almonds, hazelnuts), etc.
• Other common allergens: sesame, buckwheat, bitter buckwheat, lysozyme and histamine, etc.
• Vegetable series: Lupin beans, celery, etc.
• Condiment series: Mustard.