英文名称
Recombinant Human DNASE1 Protein, N-His
英文别名
DNL1; Deoxyribonuclease I; DNASE1; Dornase alfa; Deoxyribonuclease-1; DNase I; DRNI
性状
Lyophilized
纯化方法
AC
理论分子量
29.35 kDa
储存液
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
研究领域
Cancer > Cell Death > Apoptosis > Metabolism

Cancer > Cell Death > Apoptosis > Nucleus > DNAses

Cell Biology > Apoptosis > Nucleus > DNAses

Epigenetics and Nuclear Signaling > Cell cycle > Apoptosis > Nuclear

Metabolism > Pathways and Processes > Metabolism processes > Apoptosis

SWISS
P24855
Gene ID
1773
活性
Not tested
序列
19-259/282
物种
Human
标签
N-His
纯度
>90% as determined by SDS-PAGE.
表达系统
E.coli
复溶
Reconstitute in sterile water for a stock solution.
保存条件
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
注意事项
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
数据库链接
Entrez Gene : 1773 Human

Omim : 125505 Human

SwissProt : P24855 Human

Unigene : 629638 Human

背景资料
Deoxyribonuclease I gene is approximately 3.2 kb long with 9 exons separated by 8 introns. In the form of a bovine pancreatic enzyme preparation, it occupies an important place in the history of protein chemistry and enzymology: it was the first enzyme to be recognized as specific for DNA; it was the first DNase to be crystallized; and it was the first DNase for which a specific protein inhibitor was characterized. DNase I is a Ca2+ and Mg2+ dependant endonuclease. DNase I is synthesized in the pancreas and stored in zymogen granules. It has been used to reduce the viscosity of cystic fibrosis sputum. A DNase I-like enzyme appears to catalyze the degradation of chromatin to oligo- and mononucleosomes during apoptosis. A recent study has demonstrated an endonuclease with activity and antigenicity indistinguishable from DNase I in thymocytes, cells susceptible to apoptosis. DNase I is an endonuclease that hydrolyzes double-stranded or single stranded DNA preferentially at sites adjacent to pyrimidine nucleotides. The product of hydrolysis is a complex mixture of 5'-phosphate mononucleotides and oligonucleotides. In the presence of Mg ion, DNase I attacks each strand of DNA independently and the cleavage sites are random.