RhoA-GTP 小鼠单抗/活性抗体使用说明书及介绍
2023-01-04 来源:本站 点击次数:1686
GTP酶是GTP/GDP结合蛋白。被细胞表面受体(GPCR)激活后, 它能调节下游效应器的功能。费斯德生物抗体，它可以结合GTP、水解GTP，因此在激活的GTP结合状态和非激活的GDP结合状态之间切换。它具有内在的GTP酶活性，可将GTP水解为GDP，从而使G蛋白失活。我们精心筛选的小鼠单克隆抗体能捕捉到与GTP结合的具有活性的GTP酶。它们已被用于通过免疫组织化学监测细胞和组织中 G 蛋白的活化和分布。
| 描述: Anti-RhoA-GTP Mouse Monoclonal Antibody |
| 背景介绍: Small GTPases are a super-family of cellular signaling regulators. RhoA belongs to the Rho sub-family of GTPases. Rho proteins play critical roles in many actin cytoskeleton- dependent processes including platelet aggregation, cell motility, contraction, and cytokinesis. It regulates the formation of stress fibers and focal adhesions in fibroblasts and Ca 2+ sensitivity of smooth muscle contraction. |
| 免疫原: Recombinant full length protein of active RhoA |
| 经过测试的应用: IP, IHC,IF |
| 推荐稀释比: IP: 1 µg for 1~2 mg total cellular proteins IHC, IF: 1:50-1:250 |
| 浓度: 1 mg/ml |
| 宿主: Mouse |
| 形式: Liquid |
| 克隆性: Monoclonal |
| 亚型: IgG2b |
| 纯度: Purified from ascites |
| 防腐剂: No |
| 成分: PBS (without Mg 2+ and Ca 2+ ), pH 7.4, 150 mM NaCl, 50% glycerol |
| 种属反应性: Anti-RhoA monoclonal antibody recognizes active RhoA from vertebrates. |
| 储存条件: Store at -20°C. Avoid repeated freezing and thawing |
| Immunoprecipitation/Western Blot: IP/WB Analysis of Active RhoA Protein. Purified recombinant RhoA proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti-RhoA-GTP monoclonal antibody (Cat. # 26904). After SDS/PAGE, the membrane filter was probed with anti-RhoA mouse monoclonal antibody (Cat. # 26007). 产品说明书和使用介绍： https://www.neweastwuhan.com/wp-content/uploads/2022/05/26904_RhoA-GTP.pdf |
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