Rap1 GTP 小鼠单抗介绍和使用说明
2023-03-16 来源:费斯德生物官网 点击次数:104Product Description
Pioneering GTPase and Oncogene Product Development since 2010
Rap1-GTP
Catalog Number: 26912
Gene Symbol: RAP1
Description: Anti-Rap1-GTP Mouse Monoclonal Antibody
Background: Small GTPases are a super-family of cellular signaling regulators. Rap family members (Rap1A, Rap1B, Rap2A, Rap2B, and Rap2C) are Ras-
like GTPases that regulate cell proliferation, differentiation, apoptosis and adhesion mechanisms. Rap1 (Ras-proximate-1) regulates apoptosis through interaction with two MAP3K effectors of the MEK/ERK MAP-kinase pathway, Raf-1 and B-Raf. Rap1 regulates cell adhesion mechanisms through effector molecules such as the integrins and cadherins.
Immunogen: Recombinant full length protein of active Rap1.
Tested Applications: IP, IHC
Recommended Dilutions:
1 g for 1~2 mg total cellular proteins for IP;
Immunoprecipitation/Western blot
Rap1 Activation Assay.
1:100 dilution for IHC
Concentration: 1 mg/ml
Host: Mouse
Clonality: Monoclonal
Isotype: IgG
Purity: Purified from ascites
Format: Liquid
Preservative: No
2+2+
Constituents: PBS (without Mg and Ca ), pH 7.4, 150 mM NaCl, 50% glycerol
Species Reactivity: Anti- Rap1-GTP antibody
recognizes active Rap1 of vertebrates.
Storage Conditions: Store at -20°C. Avoid freeze / thaw cycles
Purified Rap1 proteins were immunoprecipitated after treated with GDP (lane 1) or GTPS (lane 2).
Immunoprecipitation was done with the anti- Rap1-
GTP monoclonal antibody (Cat. No. 26912).
Immunoblot was with an anti-Rap1 polyclonal
antibody (Cat. No. 21104)
FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC APPLICATIONS
NewEast Biosciences
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