微射流纳米均质机用于细胞破碎
2020-02-19 来源:国外 点击次数:555
Cell Disruption Application Charts
| Sample | PSI | Passes | Results |
| Meningicoccal cell paste (4%) in buffer |
1000 | 1 | Cooling used to maintain 10C temperature. Complete rupture achieved in one pass. |
| Mammalian cells | 2000 | 1 | Complete rupture of cells and easy separation of parasite within the cell achieved in one pass on laboratory model H-5000. |
| E. coli (10%) in buffer | 6000 | 3 | 90% rupture achieved. Results scaled linearly from lab to production. |
| 10000 | 1 | 90% rupture achieved. | |
| 10000 | 3 | 90% rupture achieved. | |
| Baker's yeast | 20000 | 10 | Cooling used. 95% rupture of yeast cells achieved. |
| Brewer's yeast (10%) in water | 10000 | 1-10 | Cooling used to keep temp below 5C. 35% rupture at 1 pass and 63% in 10 passes. |
| 20000 | 1-10 | 71% rupture at 1 pass and 98% in 10 passes. Yield of enzyme significantly better than that achieved with other processing techniques including French press. |
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